Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)

Overview

  • Product name
    Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388]
    See all PI 3 Kinase catalytic subunit gamma primary antibodies
  • Description
    Rabbit monoclonal [Y388] to PI 3 Kinase catalytic subunit gamma
  • Specificity
    This antibody is specific for PI 3 Kinase catalytic subunit gamma.
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the N terminal of Human PI 3 Kinase catalytic subunit gamma.

  • Epitope
    ab32089 reacts with an epitope located in the N terminal region of PI 3 Kinase catalytic subunit gamma
  • Positive control
    • Jurkat cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 126 kDa.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function
      3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
    • Tissue specificity
      Pancreas, skeletal muscle, liver and heart.
    • Pathway
      Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similarities
      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidylinositol 3 kinase antibody
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
      • 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
      • p110 gamma antibody
      • p110gamma antibody
      • p120 PI3K antibody
      • p120-PI3K antibody
      • Phosphatidylinositol 3 kinase catalytic 110 kD gamma antibody
      • Phosphatidylinositol 3 kinase gamma, p110 gamma antibody
      • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase catalytic gamma polypeptide antibody
      • Phosphoinositide 3 kinase gamma catalytic subunit antibody
      • PI3 kinase p110 subunit gamma antibody
      • PI3-kinase subunit gamma antibody
      • PI3CG antibody
      • PI3K antibody
      • PI3K-gamma antibody
      • PI3Kgamma antibody
      • PIK3 antibody
      • Pik3cg antibody
      • PK3CG_HUMAN antibody
      • PtdIns-3-kinase subunit gamma antibody
      • PtdIns-3-kinase subunit p110-gamma antibody
      • Serine/threonine protein kinase PIK3CG antibody
      see all

    Images

    • Overlay histogram showing HeLa cells stained with ab32089 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32089, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089) at 1/1000 dilution + Jurkat cell lysate.

      Predicted band size : 126 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Conte E  et al. PI3K p110? overexpression in idiopathic pulmonary fibrosis lung tissue and fibroblast cells: in vitro effects of its inhibition. Lab Invest 93:566-76 (2013). Human . Read more (PubMed: 23439433) »
    • Conte E  et al. Inhibition of PI3K prevents the proliferation and differentiation of human lung fibroblasts into myofibroblasts: the role of class I P110 isoforms. PLoS One 6:e24663 (2011). WB ; Human . Read more (PubMed: 21984893) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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