RabMAb

Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)

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Overview

  • Product nameAnti-PI 3 Kinase catalytic subunit gamma antibody [Y388]
    See all PI 3 Kinase catalytic subunit gamma primary antibodies
  • Description
    Rabbit monoclonal [Y388] to PI 3 Kinase catalytic subunit gamma
  • SpecificityThis antibody is specific for PI 3 Kinase catalytic subunit gamma.
  • Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the N terminal of Human PI 3 Kinase catalytic subunit gamma.

  • Epitopeab32089 reacts with an epitope located in the N terminal region of PI 3 Kinase catalytic subunit gamma
  • Positive control
    • Jurkat cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 126 kDa.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
  • Application notesIs unsuitable for ICC or IHC.

    • Target

    • Function3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
    • Tissue specificityPancreas, skeletal muscle, liver and heart.
    • PathwayPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Information by UniProt
    • Database links
      • Alternative names
        • 1 phosphatidylinositol 3 kinase antibody
        • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
        • 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
        • p110 gamma antibody
        • p110gamma antibody
        • p120 PI3K antibody
        • p120-PI3K antibody
        • Phosphatidylinositol 3 kinase catalytic 110 kD gamma antibody
        • Phosphatidylinositol 3 kinase gamma, p110 gamma antibody
        • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide antibody
        • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma antibody
        • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma antibody
        • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform antibody
        • Phosphatidylinositol-4 antibody
        • Phosphoinositide 3 kinase catalytic gamma polypeptide antibody
        • Phosphoinositide 3 kinase gamma catalytic subunit antibody
        • PI3 kinase p110 subunit gamma antibody
        • PI3-kinase subunit gamma antibody
        • PI3CG antibody
        • PI3K antibody
        • PI3K-gamma antibody
        • PI3Kgamma antibody
        • PIK3 antibody
        • Pik3cg antibody
        • PK3CG_HUMAN antibody
        • PtdIns-3-kinase subunit gamma antibody
        • PtdIns-3-kinase subunit p110-gamma antibody
        • Serine/threonine protein kinase PIK3CG antibody
        see all

      Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] images

      • Flow Cytometry - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)
        Flow Cytometry - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)
        Overlay histogram showing HeLa cells stained with ab32089 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32089, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
      • Western blot - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)
        Western blot - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)
        Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089) at 1/1000 dilution + Jurkat cell lysate.

        Predicted band size : 126 kDa
        Observed band size : 110 kDa (why is the actual band size different from the predicted?)

      References for Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)

      This product has been referenced in:
      • Conte E  et al. PI3K p110? overexpression in idiopathic pulmonary fibrosis lung tissue and fibroblast cells: in vitro effects of its inhibition. Lab Invest 93:566-76 (2013). Human . Read more (PubMed: 23439433) »
      • Conte E  et al. Inhibition of PI3K prevents the proliferation and differentiation of human lung fibroblasts into myofibroblasts: the role of class I P110 isoforms. PLoS One 6:e24663 (2011). WB ; Human . Read more (PubMed: 21984893) »

      See all 2 Publications for this product

      Publishing research using ab32089? Please let us know so that we can cite the reference in this datasheet.

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