Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-PI 3 Kinase catalytic subunit gamma antibody [Y388]
See all PI 3 Kinase catalytic subunit gamma primary antibodies -
DescriptionRabbit monoclonal [Y388] to PI 3 Kinase catalytic subunit gamma
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Host speciesRabbit
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SpecificityThis antibody is specific for PI 3 Kinase catalytic subunit gamma.
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Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC or IHC -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to residues in the N terminal of Human PI 3 Kinase catalytic subunit gamma.
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Epitopeab32089 reacts with an epitope located in the N terminal region of PI 3 Kinase catalytic subunit gamma
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Positive control
- Jurkat cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberY388
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab32089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 126 kDa. | |
| Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/50. |
Application notes
Is unsuitable for ICC or IHC.
Target
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Function3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
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Tissue specificityPancreas, skeletal muscle, liver and heart.
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PathwayPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
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Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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Database links
- Entrez Gene: 5294 Human
- GenBank: AF327656 Human
- Omim: 601232 Human
- SwissProt: P48736 Human
- Unigene: 32942 Human
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Alternative names
- 1 phosphatidylinositol 3 kinase antibody
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
- 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
see all
Images
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![Flow Cytometry - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)](http://a.abcam.com/ps/products/32/ab32089/Images/ab32089-2-ab32089FC.jpg)
Flow Cytometry - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)Overlay histogram showing HeLa cells stained with ab32089 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32089, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Western blot - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)](http://a.abcam.com/ps/products/32/ab32089/Images/ab32089.jpg)
Western blot - Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089) at 1/1000 dilution + Jurkat cell lysate.
Predicted band size: 126 kDa
Observed band size: 110 kDa (why is the actual band size different from the predicted?)
References
This product has been referenced in:
- Conte E et al. PI3K p110? overexpression in idiopathic pulmonary fibrosis lung tissue and fibroblast cells: in vitro effects of its inhibition. Lab Invest 93:566-76 (2013). Human . Read more (PubMed: 23439433) »
- Conte E et al. Inhibition of PI3K prevents the proliferation and differentiation of human lung fibroblasts into myofibroblasts: the role of class I P110 isoforms. PLoS One 6:e24663 (2011). WB ; Human . Read more (PubMed: 21984893) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"