Validated using a knockout cell line
Recombinant
RabMAb

Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

Overview

  • Product name
    Anti-PI 3 Kinase p110 delta antibody [EPR386]
    See all PI 3 Kinase p110 delta primary antibodies
  • Description
    Rabbit monoclonal [EPR386] to PI 3 Kinase p110 delta
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human PI3 Kinase p110 delta.

  • Positive control
    • Jurkat, Raji, K562, RAW 264.7, Rat brain, Rat kidney and Rat spleen lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-PI 3 Kinase p110 delta antibody (HRP) [EPR386] (ab200773)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 119 kDa.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Tissue specificity
      Expressed predominantly in leukocytes.
    • Pathway
      Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similarities
      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
      • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
      • APDS antibody
      • GRB1 antibody
      • IMD14 antibody
      • p110d antibody
      • p110delta antibody
      • p110dp85a antibody
      • p85-ALPHA antibody
      • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
      • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase B antibody
      • Phosphoinositide 3 kinase C antibody
      • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
      • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
      • PI3 kinase p110 subunit delta antibody
      • PI3-kinase subunit delta antibody
      • PI3K antibody
      • PI3K-delta antibody
      • PI3Kdelta antibody
      • Pik3cd antibody
      • PIK3R1 antibody
      • PK3CD antibody
      • PK3CD_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns 3 kinase subunit p110 delta antibody
      • PtdIns-3-kinase subunit delta antibody
      • PtdIns-3-kinase subunit p110-delta antibody
      see all

    Anti-PI 3 Kinase p110 delta antibody [EPR386] images



    • Predicted band size : 119 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

      Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: PIK3CD knockout HAP1 whole cell lysate (40 µg)
      Lane 3: Jurkat whole cell lysate (40 µg)
      Lane 4: K562 whole cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109006 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109006 was shown to specifically react with PIK3CD when PIK3CD knockout samples were used. Wild-type and PIK3CD knockout samples were subjected to SDS-PAGE. Ab109006 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : Rat brain tissue lysate
      Lane 6 : Rat kidney tissue lysate
      Lane 7 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 119 kDa

    References for Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

    This product has been referenced in:
    • Li Q  et al. Phosphoinositide 3-kinase p110d mediates estrogen- and FSH-stimulated ovarian follicle growth. Mol Endocrinol 27:1468-82 (2013). Read more (PubMed: 23820902) »
    • Le Floc'h A  et al. Annular PIP3 accumulation controls actin architecture and modulates cytotoxicity at the immunological synapse. J Exp Med 210:2721-37 (2013). Read more (PubMed: 24190432) »

    See all 2 Publications for this product

    Product Wall

    That lot is also 6.8 mg/ml. Any vial labeled GR42675 will be that concentration. The number after, for instance -6 or -7, is a reference number that identifies the date the antibody was aliquotted into the vial. Please let me know if you have other que...

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    Thank you for contacting us.

    The IgG concentration of the lot you have, GR42675-7, is 6.8 mg/ml.

    For an isotype control, I recommend antibody ab37415:

    Click here (or use the following: http://www.abcam.com/index.html?da...

    Read More

    Thank you for contacting us.
    I am sorry to hear that the antibodies for PI3K p110 delta do not seem to work as expected.

    As we discussed over the phone, I would like to suggest to try the following:

    1) Load moreprotein onto th...

    Read More

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab109006. To check the status of the order please contact our Custome...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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