Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401)

Overview

  • Product nameAnti-PI 3 Kinase p110 delta antibody [Y387]
    See all PI 3 Kinase p110 delta primary antibodies
  • Description
    Rabbit monoclonal [Y387] to PI 3 Kinase p110 delta
  • Specificityab32401 recognises PI3-Kinases (PI3-Ks).
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide of human PI3-K p110 delta.

  • Positive control
    • Jurkat cell lysate and HeLa cells.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 119 kDa).
ICC/IF 1/250 - 1/500.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt or IHC.
  • Target

    • Tissue specificityExpressed predominantly in leukocytes.
    • PathwayPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
      • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
      • APDS antibody
      • GRB1 antibody
      • IMD14 antibody
      • p110d antibody
      • p110delta antibody
      • p110dp85a antibody
      • p85-ALPHA antibody
      • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
      • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase B antibody
      • Phosphoinositide 3 kinase C antibody
      • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
      • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
      • PI3 kinase p110 subunit delta antibody
      • PI3-kinase subunit delta antibody
      • PI3K antibody
      • PI3K-delta antibody
      • PI3Kdelta antibody
      • Pik3cd antibody
      • PIK3R1 antibody
      • PK3CD antibody
      • PK3CD_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns 3 kinase subunit p110 delta antibody
      • PtdIns-3-kinase subunit delta antibody
      • PtdIns-3-kinase subunit p110-delta antibody
      see all

    Anti-PI 3 Kinase p110 delta antibody [Y387] images

    • Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401) at 1/500 dilution + Jurkat cell lysate

      Predicted band size : 119 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab32401 at 1/250 dilution.

    References for Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401)

    This product has been referenced in:
    • Milara J  et al. Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients. Respir Res 17:145 (2016). IP ; Human . Read more (PubMed: 27825347) »
    • Mendizabal-Zubiaga J  et al. Cannabinoid CB1 Receptors Are Localized in Striated Muscle Mitochondria and Regulate Mitochondrial Respiration. Front Physiol 7:476 (2016). IP ; Mouse . Read more (PubMed: 27826249) »

    See all 8 Publications for this product

    Product Wall

    Thank you for contacting us.
    I am sorry to hear that the antibodies for PI3K p110 delta do not seem to work as expected.

    As we discussed over the phone, I would like to suggest to try the following:

    1) Load moreprotein onto th...

    Read More

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab109006. To check the status of the order please contact our Custome...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"