Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401)

Overview

  • Product nameAnti-PI 3 Kinase p110 delta antibody [Y387]
    See all PI 3 Kinase p110 delta primary antibodies
  • Description
    Rabbit monoclonal [Y387] to PI 3 Kinase p110 delta
  • Specificityab32401 recognises PI3-Kinases (PI3-Ks).
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide of human PI3-K p110 delta.

  • Positive control
    • Jurkat cell lysate and HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 119 kDa).
ICC/IF 1/250 - 1/500.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt or IHC.
  • Target

    • Tissue specificityExpressed predominantly in leukocytes.
    • PathwayPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
      • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
      • APDS antibody
      • GRB1 antibody
      • IMD14 antibody
      • p110d antibody
      • p110delta antibody
      • p110dp85a antibody
      • p85-ALPHA antibody
      • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
      • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase B antibody
      • Phosphoinositide 3 kinase C antibody
      • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
      • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
      • PI3 kinase p110 subunit delta antibody
      • PI3-kinase subunit delta antibody
      • PI3K antibody
      • PI3K-delta antibody
      • PI3Kdelta antibody
      • Pik3cd antibody
      • PIK3R1 antibody
      • PK3CD antibody
      • PK3CD_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns 3 kinase subunit p110 delta antibody
      • PtdIns-3-kinase subunit delta antibody
      • PtdIns-3-kinase subunit p110-delta antibody
      see all

    Anti-PI 3 Kinase p110 delta antibody [Y387] images

    • Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401) at 1/500 dilution + Jurkat cell lysate

      Predicted band size : 119 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab32401 at 1/250 dilution.

    References for Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401)

    This product has been referenced in:
    • Conte E  et al. PI3K p110? overexpression in idiopathic pulmonary fibrosis lung tissue and fibroblast cells: in vitro effects of its inhibition. Lab Invest 93:566-76 (2013). WB ; Human . Read more (PubMed: 23439433) »
    • Law AJ  et al. Neuregulin 1-ErbB4-PI3K signaling in schizophrenia and phosphoinositide 3-kinase-p110d inhibition as a potential therapeutic strategy. Proc Natl Acad Sci U S A 109:12165-70 (2012). Read more (PubMed: 22689948) »

    See all 6 Publications for this product

    Product Wall

    Thank you for contacting us.
    I am sorry to hear that the antibodies for PI3K p110 delta do not seem to work as expected.

    As we discussed over the phone, I would like to suggest to try the following:

    1) Load moreprotein onto th...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab109006. To check the status of the order please contact our Custome...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"