Anti-PI 3 Kinase p55 gamma antibody (ab76460)

Overview

  • Product nameAnti-PI 3 Kinase p55 gamma antibody
    See all PI 3 Kinase p55 gamma primary antibodies
  • Description
    Mouse monoclonal to PI 3 Kinase p55 gamma
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 271-381 of Human PI 3 Kinase p55 gamma (AAH21622) with tag.

  • Positive control
    • Recombinant tagged PI 3 Kinase p55 gamma fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 54 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
    • Tissue specificityHighest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
    • Sequence similaritiesBelongs to the PI3K p85 subunit family.
      Contains 2 SH2 domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686P05226 antibody
      • FLJ41892 antibody
      • OTTHUMP00000009783 antibody
      • OTTHUMP00000009786 antibody
      • p55 antibody
      • p55 gamma antibody
      • P55G_HUMAN antibody
      • p55PIK antibody
      • Phosphatidylinositol 3 kinase regulatory subunit gamma antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody
      • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
      • Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody
      • Phosphatidylinositol 3-kinase regulatory subunit gamma antibody
      • Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody
      • Phosphoinositide 3 kinase regulatory subunit 3 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody
      • Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) antibody
      • Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
      • PI3 kinase p85 subunit gamma antibody
      • PI3-kinase regulatory subunit gamma antibody
      • PI3-kinase subunit p55-gamma antibody
      • PI3K regulatory subunit gamma antibody
      • Pik3r3 antibody
      • PtdIns 3 kinase p85 gamma antibody
      • PtdIns-3-kinase regulatory subunit gamma antibody
      • PtdIns-3-kinase regulatory subunit p55-gamma antibody
      see all

    Anti-PI 3 Kinase p55 gamma antibody images

    • Anti-PI 3 Kinase p55 gamma antibody (ab76460) at 1 µg/ml + Recombinant tagged PI 3 Kinase p55 gamma fragment at 0.1 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 54 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-PI 3 Kinase p55 gamma antibody (ab76460)

    ab76460 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76460.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"