Anti-PI 3 Kinase p55 gamma antibody (ab97862)

Overview

  • Product name
    Anti-PI 3 Kinase p55 gamma antibody
    See all PI 3 Kinase p55 gamma primary antibodies
  • Description
    Rabbit polyclonal to PI 3 Kinase p55 gamma
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 242-437 of Human PI 3 Kinase p55 gamma (AAH21622).

  • Positive control
    • 293T, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates; A431 whole cell lysate. SAS xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.

Target

  • Function
    Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
  • Tissue specificity
    Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
  • Sequence similarities
    Belongs to the PI3K p85 subunit family.
    Contains 2 SH2 domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686P05226 antibody
    • FLJ41892 antibody
    • OTTHUMP00000009783 antibody
    • OTTHUMP00000009786 antibody
    • p55 antibody
    • p55 gamma antibody
    • P55G_HUMAN antibody
    • p55PIK antibody
    • Phosphatidylinositol 3 kinase regulatory subunit gamma antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
    • Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody
    • Phosphatidylinositol 3-kinase regulatory subunit gamma antibody
    • Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody
    • Phosphoinositide 3 kinase regulatory subunit 3 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody
    • Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) antibody
    • Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody
    • PI3 kinase p85 subunit gamma antibody
    • PI3-kinase regulatory subunit gamma antibody
    • PI3-kinase subunit p55-gamma antibody
    • PI3K regulatory subunit gamma antibody
    • Pik3r3 antibody
    • PtdIns 3 kinase p85 gamma antibody
    • PtdIns-3-kinase regulatory subunit gamma antibody
    • PtdIns-3-kinase regulatory subunit p55-gamma antibody
    see all

Images

  • Anti-PI 3 Kinase p55 gamma antibody (ab97862) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 54 kDa
  • ab97862 at 1/500 dilution staining PI 3 Kinase p55 gamma in SAS xenograft by Immunohistochemistry, Paraffin-embedded tissue.

References

ab97862 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Rat Cell (Dorsal root ganglion neurons)
Specification
Dorsal root ganglion neurons
Permeabilization
Yes - .1% triton-x was used in phosphate buffered saline washes
Fixative
Paraformaldehyde
Username

Rory Pritchard

Verified customer

Submitted Dec 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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