Anti-PI 3 Kinase p85 alpha antibody (ab65261)


  • Product nameAnti-PI 3 Kinase p85 alpha antibody
    See all PI 3 Kinase p85 alpha primary antibodies
  • Description
    Rabbit polyclonal to PI 3 Kinase p85 alpha
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y368 of human PIK3R1.

  • Positive control
    • 293 cell lysate.


Associated products


Our Abpromise guarantee covers the use of ab65261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
ELISA 1/1000.


  • FunctionBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • Tissue specificityIsoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
  • Sequence similaritiesBelongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
  • Post-translational
    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt
  • Database links
  • Alternative names
    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Anti-PI 3 Kinase p85 alpha antibody images

  • All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab65261) at 1/50 dilution

    Lane 1 : 293 cell lysate, nontransfected
    Lane 2 : 293 cell lysate, transiently transfected with the PIK3R1 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa

References for Anti-PI 3 Kinase p85 alpha antibody (ab65261)

ab65261 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65261.
Please use the links above to contact us or submit feedback about this product.