Anti-PI 3 Kinase p85 alpha antibody (ab71522)


  • Product nameAnti-PI 3 Kinase p85 alpha antibody
    See all PI 3 Kinase p85 alpha primary antibodies
  • Description
    Rabbit polyclonal to PI 3 Kinase p85 alpha
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y580 of human PI 3 Kinase p85 alpha

  • Positive control
    • Transiently transfected 293 cell lysate and human skeletal muscle tissue



Our Abpromise guarantee covers the use of ab71522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).


  • FunctionBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • Tissue specificityIsoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
  • Sequence similaritiesBelongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
  • Post-translational
    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt
  • Database links
  • Alternative names
    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Anti-PI 3 Kinase p85 alpha antibody images

  • All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab71522) at 1/50 dilution

    Lane 1 : Non transfected 293 cell line lysate
    Lane 2 : 293 cell line lysate transiently transfected with the Pl 3 Kinase p85 alpha gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
  • ab71522, at a 1/10 dilution, staining Pl 3 Kinase p85 alpha in formalin fixed, paraffin embedded human skeletal muscle tissue by Immunohistochemistry. ab71522 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-PI 3 Kinase p85 alpha antibody (ab71522)

ab71522 has not yet been referenced specifically in any publications.

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