Anti-PI 3 Kinase p85 alpha antibody [U13] (ab250)

Overview

  • Product nameAnti-PI 3 Kinase p85 alpha antibody [U13]
    See all PI 3 Kinase p85 alpha primary antibodies
  • Description
    Mouse monoclonal [U13] to PI 3 Kinase p85 alpha
  • SpecificityRecognises the SH3 domain of the p85 alpha subunit of bovine PI 3 kinase. This antibody is specific for the p85 alpha isoform and does not bind to the p85 beta isoform (see End et al, J Biol Chem. 1993 May 15;268(14):10066-75).
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Monkey
  • Immunogen

    Baculovirus expressed (recombinant) Bovine p85 alpha subunit of PI 3 Kinase.

Properties

Applications

Our Abpromise guarantee covers the use of ab250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200.
IP Use at an assay dependent dilution. USE NEAT.

Target

  • FunctionBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • Tissue specificityIsoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
  • Sequence similaritiesBelongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
  • Post-translational
    modifications
    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt
  • Database links
  • Alternative names
    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

References for Anti-PI 3 Kinase p85 alpha antibody [U13] (ab250)

This product has been referenced in:
  • Rabinovsky R  et al. p85 Associates with unphosphorylated PTEN and the PTEN-associated complex. Mol Cell Biol 29:5377-88 (2009). WB ; Human . Read more (PubMed: 19635806) »
  • Salazar L  et al. A novel interaction between fibroblast growth factor receptor 3 and the p85 subunit of phosphoinositide 3-kinase: activation-dependent regulation of ERK by p85 in multiple myeloma cells. Hum Mol Genet 18:1951-61 (2009). WB ; Human . Read more (PubMed: 19286672) »

See all 4 Publications for this product

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