The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
1/50 - 1/100.
Use a concentration of 5 µg/ml.
FunctionRegulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Involvement in diseaseMegalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
Sequence similaritiesBelongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.
DomainThe SH2 2 domain is required for interaction with FBXL2 and PTPN13.
Post-translational modificationsPhosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317). Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
ICC/IF image of ab62901 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62901 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab62901 at 1/50 - 1/100 dilution, staining PI 3 Kinase p85 beta in human brain by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue. ab62901 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot - PI 3 Kinase p85 beta antibody (ab62901)
All lanes : Anti-PI 3 Kinase p85 beta antibody (ab62901) at 1/100 dilution
Lane 1 : 293 cell lysates, nontransfected Lane 2 : 293 cell lysates, transiently transfected with the PI 3 Kinase p85 beta gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 82 kDa Observed band size : 82 kDa Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-PI 3 Kinase p85 beta antibody (ab62901)
has not yet been referenced specifically in any publications.
Publishing research using ab62901? Please let us know so that we can cite the reference in this datasheet.
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