Anti-PI 3 Kinase p85 beta antibody (ab93778)

Overview

  • Product nameAnti-PI 3 Kinase p85 beta antibody
    See all PI 3 Kinase p85 beta primary antibodies
  • Description
    Rabbit polyclonal to PI 3 Kinase p85 beta
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region within amino acids 75 - 125 of Human PI 3 Kinase p85 beta (NP_005018.1)

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 5-15µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
    • Involvement in diseaseMegalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
    • Sequence similaritiesBelongs to the PI3K p85 subunit family.
      Contains 1 Rho-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • DomainThe SH2 2 domain is required for interaction with FBXL2 and PTPN13.
    • Post-translational
      modifications
      Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
      Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
    • Information by UniProt
    • Database links
    • Alternative names
      • p85 antibody
      • p85 beta antibody
      • P85B antibody
      • P85B_HUMAN antibody
      • Phosphatidylinositol 3 kinase antibody
      • Phosphatidylinositol 3 kinase regulatory beta subunit antibody
      • Phosphatidylinositol 3 kinase regulatory subunit beta antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
      • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody
      • Phosphatidylinositol 3-kinase regulatory subunit beta antibody
      • Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody
      • Phosphoinositide 3 kinase regulatory subunit 2 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody
      • Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody
      • Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody
      • PI3 kinase p85 beta subunit antibody
      • PI3 kinase p85 subunit beta antibody
      • PI3-kinase regulatory subunit beta antibody
      • PI3-kinase subunit p85-beta antibody
      • PI3K antibody
      • PI3K regulatory subunit beta antibody
      • PIK3R 2 antibody
      • PIK3R2 antibody
      • PtdIns 3 kinase p85 beta antibody
      • PtdIns-3-kinase regulatory subunit beta antibody
      • PtdIns-3-kinase regulatory subunit p85-beta antibody
      see all

    Anti-PI 3 Kinase p85 beta antibody images

    • All lanes : Anti-PI 3 Kinase p85 beta antibody (ab93778) at 0.04 µg/ml

      Lane 1 : Hela cell lysate at 50 µg
      Lane 2 : Hela cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 82 kDa


      Exposure time : 30 seconds
    • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane
      All lanes : PI 3 Kinase p85 beta antibody (ab93778) at 1 µg/ml
      Lane 1 : Lysate immunoprecipitated with ab93778
      Lane 2 : Lysate immunoprecipitated with a control IgG
      Predicted band size : 82 kDa
      Developed using the ECL technique
      Exposure time : 10 seconds

    References for Anti-PI 3 Kinase p85 beta antibody (ab93778)

    ab93778 has not yet been referenced specifically in any publications.

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