The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 5-15µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.
The SH2 2 domain is required for interaction with FBXL2 and PTPN13.
Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317). Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
Western blot - PI 3 Kinase p85 beta antibody (ab93778)
All lanes : Anti-PI 3 Kinase p85 beta antibody (ab93778) at 0.04 µg/ml
Lane 1 : Hela cell lysate at 50 µg Lane 2 : Hela cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 30 seconds
Immunoprecipitation - PI 3 Kinase p85 beta antibody (ab93778)
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane All lanes : PI 3 Kinase p85 beta antibody (ab93778) at 1 µg/ml Lane 1 : Lysate immunoprecipitated with ab93778 Lane 2 : Lysate immunoprecipitated with a control IgG Predicted band size : 82 kDa
Developed using the ECL technique Exposure time : 10 seconds