Overview

  • Product nameAnti-PI 3 Kinase p85 beta antibody [T15]
    See all PI 3 Kinase p85 beta primary antibodies
  • Description
    Mouse monoclonal [T15] to PI 3 Kinase p85 beta
  • SpecificityT15 recognises the p85 beta subunit of bovine PI 3 kinase. It does not cross react with the p85 alpha subunit.
  • Tested applicationsSuitable for: IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Predicted to work with: Mouse, Rat
  • Immunogen

    Balculovirus expressed (Recombinant) bovine P85 beta subunit of PI 3 kinase.

  • Positive control
    • Lysates of SF9 cells infected with baculovirus expressing p85 beta.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone numberT15
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP 1/25. Use 25ul.
WB 1/1000 - 1/5000. Predicted molecular weight: 82 kDa.

Target

  • FunctionRegulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
  • Involvement in diseaseMegalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
  • Sequence similaritiesBelongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • DomainThe SH2 2 domain is required for interaction with FBXL2 and PTPN13.
  • Post-translational
    modifications
    Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
    Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
  • Information by UniProt
  • Database links
  • Alternative names
    • p85 antibody
    • p85 beta antibody
    • P85B antibody
    • P85B_HUMAN antibody
    • Phosphatidylinositol 3 kinase antibody
    • Phosphatidylinositol 3 kinase regulatory beta subunit antibody
    • Phosphatidylinositol 3 kinase regulatory subunit beta antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody
    • Phosphatidylinositol 3-kinase regulatory subunit beta antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody
    • PI3 kinase p85 beta subunit antibody
    • PI3 kinase p85 subunit beta antibody
    • PI3-kinase regulatory subunit beta antibody
    • PI3-kinase subunit p85-beta antibody
    • PI3K antibody
    • PI3K regulatory subunit beta antibody
    • PIK3R 2 antibody
    • PIK3R2 antibody
    • PtdIns 3 kinase p85 beta antibody
    • PtdIns-3-kinase regulatory subunit beta antibody
    • PtdIns-3-kinase regulatory subunit p85-beta antibody
    see all

Anti-PI 3 Kinase p85 beta antibody [T15] images



  • Predicted band size : 82 kDa

References for Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)

This product has been referenced in:
  • Du C  et al. MiR-126-3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2. J Transl Med 12:259 (2014). Human . Read more (PubMed: 25240815) »
  • Ketscher A  et al. LSD1 controls metastasis of androgen-independent prostate cancer cells through PXN and LPAR6. Oncogenesis 3:e120 (2014). WB, IP ; Human . Read more (PubMed: 25285406) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab28356.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"