Overview

  • Product name
    Anti-PI 3 Kinase p85 beta antibody [T15]
    See all PI 3 Kinase p85 beta primary antibodies
  • Description
    Mouse monoclonal [T15] to PI 3 Kinase p85 beta
  • Specificity
    T15 recognises the p85 beta subunit of bovine PI 3 kinase. It does not cross react with the p85 alpha subunit.
  • Tested applications
    Suitable for: IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Predicted to work with: Mouse, Rat
  • Immunogen

    Balculovirus expressed (Recombinant) bovine P85 beta subunit of PI 3 kinase.

  • Positive control
    • Lysates of SF9 cells infected with baculovirus expressing p85 beta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    T15
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP 1/25. Use 25ul.
WB 1/1000 - 1/5000. Predicted molecular weight: 82 kDa.

Target

  • Function
    Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
  • Involvement in disease
    Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
  • Sequence similarities
    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain
    The SH2 2 domain is required for interaction with FBXL2 and PTPN13.
  • Post-translational
    modifications
    Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
    Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
  • Information by UniProt
  • Database links
  • Alternative names
    • p85 antibody
    • p85 beta antibody
    • P85B antibody
    • P85B_HUMAN antibody
    • Phosphatidylinositol 3 kinase antibody
    • Phosphatidylinositol 3 kinase regulatory beta subunit antibody
    • Phosphatidylinositol 3 kinase regulatory subunit beta antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody
    • Phosphatidylinositol 3-kinase regulatory subunit beta antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody
    • PI3 kinase p85 beta subunit antibody
    • PI3 kinase p85 subunit beta antibody
    • PI3-kinase regulatory subunit beta antibody
    • PI3-kinase subunit p85-beta antibody
    • PI3K antibody
    • PI3K regulatory subunit beta antibody
    • PIK3R 2 antibody
    • PIK3R2 antibody
    • PtdIns 3 kinase p85 beta antibody
    • PtdIns-3-kinase regulatory subunit beta antibody
    • PtdIns-3-kinase regulatory subunit p85-beta antibody
    see all

Images



  • Predicted band size : 82 kDa

References

This product has been referenced in:
  • Felli N  et al. AP2a controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression. Oncogene 35:3016-26 (2016). WB . Read more (PubMed: 26434590) »
  • Du C  et al. MiR-126-3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2. J Transl Med 12:259 (2014). Human . Read more (PubMed: 25240815) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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