Anti-PI 3 Kinase p85 beta antibody [T4] (ab251)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-PI 3 Kinase p85 beta antibody [T4]
See all PI 3 Kinase p85 beta primary antibodies - DescriptionMouse monoclonal [T4] to PI 3 Kinase p85 beta
- SpecificityT4 is specific for the p85 beta subunit of bovine PI 3 kinase.
- Tested applicationsSuitable for: WB, IP, IHC-Frmore details
- Species reactivityReacts with: Cow, Human, Monkey
- Immunogen
Baculovirus expressed (recombinant) Bovine p85 beta subunit of PI 3 Kinase.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 5-10% Foetal Calf Serum, 0.2M Tris HCl. pH 7.4 - PurityTissue culture supernatant
- ClonalityMonoclonal
- Clone numberT4
- MyelomaNS1
- IsotypeIgG1
- Light chain typeunknown
- Research areas
Applications
Our Abpromise guarantee covers the use of ab251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50 - 1/100. | |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
- FunctionRegulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
- Involvement in diseaseMegalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
- Sequence similaritiesBelongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. - DomainThe SH2 2 domain is required for interaction with FBXL2 and PTPN13.
- Post-translational
modificationsPhosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
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Database links
- Entrez Gene: 282308 Cow
- Entrez Gene: 5296 Human
- Omim: 603157 Human
- SwissProt: P23726 Cow
- SwissProt: O00459 Human
- Unigene: 371344 Human
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Alternative names
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
References for Anti-PI 3 Kinase p85 beta antibody [T4] (ab251)
This product has been referenced in:
- Salazar L et al. A novel interaction between fibroblast growth factor receptor 3 and the p85 subunit of phosphoinositide 3-kinase: activation-dependent regulation of ERK by p85 in multiple myeloma cells. Hum Mol Genet 18:1951-61 (2009). WB ; Human . Read more (PubMed: 19286672) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"