Overview

  • Product name
    Anti-PI 3 Kinase p85 gamma antibody
    See all PI 3 Kinase p85 gamma primary antibodies
  • Description
    Rabbit polyclonal to PI 3 Kinase p85 gamma
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: LQEYHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC, corresponding to internal sequence amino acids 181-230 of Human PI 3 Kinase p85 gamma

  • Positive control
    • Transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    PI3 Kinase p85 gamma (PIK3R3) binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is an heterodimer of a p110 (catalytic) and a p55 (regulatory) subunits. It is expressed at highest levels in brain and testis; at lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • p55 GAMMA antibody
    • p55PIK antibody
    • Phosphatidylinositol 3 kinase 55 kDa regulatory subunit gamma antibody
    • Phosphatidylinositol 3 kinase regulatory subunit gamma antibody
    • PI3 kinase regulatory subunit gamma antibody
    • PI3 kinase subunit p55 gamma antibody
    • PI3K regulatory subunit gamma antibody
    • PIK3R3 antibody
    • PtdIns 3 kinase p85 gamma antibody
    • PtdIns 3 kinase regulatory subunit p55 gamma antibody
    • PtdIns-3 kinase regulatory subunit gamma antibody
    see all

Images

  • Anti-PI 3 Kinase p85 gamma antibody (ab69870) at 5 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 44 kDa (possible isoform).

References

This product has been referenced in:
  • Zaman MS  et al. Inhibition of PTEN gene expression by oncogenic miR-23b-3p in renal cancer. PLoS One 7:e50203 (2012). WB ; Human . Read more (PubMed: 23189187) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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