Recombinant Anti-VPS15 antibody [EPR5303(2)] (ab124817)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5303(2)] to VPS15
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VPS15 antibody [EPR5303(2)]
See all VPS15 primary antibodies -
Description
Rabbit monoclonal [EPR5303(2)] to VPS15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji cell lysate, Jurkat cell lysate, Molt-4 cell lysate, 293T cell lysate, C6 cell lysate and Raw 264.7 cell lysate. Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5303(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Contains 7 WD repeats. -
Post-translational
modificationsMyristoylated.
Probably autophosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 30849 Human
- Entrez Gene: 75669 Mouse
- Entrez Gene: 363131 Rat
- Omim: 602610 Human
- SwissProt: Q99570 Human
- SwissProt: Q8VD65 Mouse
- SwissProt: P0C0R5 Rat
- Unigene: 149032 Human
see all -
Alternative names
- MGC102700 antibody
- P150 antibody
- Phosphatidylinositol 3 kinase associated p150 antibody
see all
Images
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All lanes : Anti-VPS15 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt-4 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Raw 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 153 kDa -
ab124817 at 1/100 dilution, staining VPS15 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124817 has been referenced in 1 publication.
- Gstrein T et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat Neurosci 21:207-217 (2018). PubMed: 29311744