Recombinant
RabMAb

Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)

Overview

  • Product name
    Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)]
    See all PI 3 Kinase regulatory subunit 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR5303(2)] to PI 3 Kinase regulatory subunit 4
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PI 3 Kinase regulatory subunit 4 aa 1300 to the C-terminus (C terminal).
    Database link: Q99570
    (Peptide available as ab183696)

  • Positive control
    • Raji cell lysate, Jurkat cell lysate, Molt-4 cell lysate, 293T cell lysate, C6 cell lysate and Raw 264.7 cell lysate. Human kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).Can be blocked with PI 3 Kinase regulatory subunit 4 peptide (ab183696).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 3 HEAT repeats.
      Contains 1 protein kinase domain.
      Contains 7 WD repeats.
    • Post-translational
      modifications
      Myristoylated.
      Probably autophosphorylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC102700 antibody
      • P150 antibody
      • Phosphatidylinositol 3 kinase associated p150 antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150 antibody
      • Phosphoinositide 3 kinase adaptor protein antibody
      • Phosphoinositide 3 kinase regulatory subunit 4 antibody
      • Phosphoinositide 3 kinase regulatory subunit 4 p150 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150 antibody
      • Phosphoinositide 3-kinase adaptor protein antibody
      • Phosphoinositide 3-kinase regulatory subunit 4 antibody
      • PI3 kinase p150 subunit antibody
      • PI3-kinase p150 subunit antibody
      • PI3-kinase regulatory subunit 4 antibody
      • PI3R4_HUMAN antibody
      • PIK3R 4 antibody
      • PIK3R4 antibody
      • VPS 15 antibody
      • VPS15 antibody
      see all

    Images

    • All lanes : Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Raw 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 153 kDa
    • ab124817 at 1/100 dilution, staining PI 3 Kinase regulatory subunit 4 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.

    References

    ab124817 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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