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Product name Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] See all PI 3 Kinase regulatory subunit 4 primary antibodies
Rabbit monoclonal [EPR5303(2)] to PI 3 Kinase regulatory subunit 4
Tested applications Suitable for:
WB, IHC-P, Flow Cyt, ICC/IF more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PI 3 Kinase regulatory subunit 4 aa 1300 to the C-terminus (C terminal).
Database link: Q99570 (Peptide available as ab183696)
Raji cell lysate, Jurkat cell lysate, Molt-4 cell lysate, 293T cell lysate, C6 cell lysate and Raw 264.7 cell lysate.
Human kidney tissue.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer pH: 7.20 Preservative: 0.01% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity Tissue culture supernatant
Clone number EPR5303(2)
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).Can be blocked with
PI 3 Kinase regulatory subunit 4 peptide (ab183696).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/500.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Application notes Is unsuitable for IP.
Function Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Tissue specificity Ubiquitously expressed.
Sequence similarities Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 3 HEAT repeats. Contains 1 protein kinase domain. Contains 7 WD repeats.
Post-translational modifications Myristoylated. Probably autophosphorylated.
Information by UniProt
Phosphatidylinositol 3 kinase associated p150 antibody
Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] images
Western blot - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
All lanes : Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution Lane 1 : Raji cell lysate Lane 2 : Jurkat cell lysate Lane 3 : Molt-4 cell lysate Lane 4 : 293T cell lysate Lane 5 : C6 cell lysate Lane 6 : Raw 264.7 cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat-anti-rabbit HRP at 1/2000 dilution Predicted band size : 153 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
ab124817 at 1/100 dilution, staining PI 3 Kinase regulatory subunit 4 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
References for Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"