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Rabbit monoclonal [EPR5303(2)] to PI 3 Kinase regulatory subunit 4
ICC/IF more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PI 3 Kinase regulatory subunit 4 aa 1300 to the C-terminus (C terminal).
Database link: Q99570 (Peptide available as ab183696)
Raji cell lysate, Jurkat cell lysate, Molt-4 cell lysate, 293T cell lysate, C6 cell lysate and Raw 264.7 cell lysate.
Human kidney tissue.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).Can be blocked with
PI 3 Kinase regulatory subunit 4 peptide (ab183696).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Is unsuitable for IP.
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats. Contains 1 protein kinase domain. Contains 7 WD repeats.
Information by UniProt
Phosphatidylinositol 3 kinase associated p150 antibody
Western blot - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
All lanes : Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution Lane 1 : Raji cell lysate Lane 2 : Jurkat cell lysate Lane 3 : Molt-4 cell lysate Lane 4 : 293T cell lysate Lane 5 : C6 cell lysate Lane 6 : Raw 264.7 cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat-anti-rabbit HRP at 1/2000 dilution Predicted band size: 153 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
ab124817 at 1/100 dilution, staining PI 3 Kinase regulatory subunit 4 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"