Anti-PI 4 Kinase type 2 beta antibody - C-terminal (ab71823)


  • Product nameAnti-PI 4 Kinase type 2 beta antibody - C-terminal
    See all PI 4 Kinase type 2 beta primary antibodies
  • Description
    Rabbit polyclonal to PI 4 Kinase type 2 beta - C-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 319-336 of Human PI 4 Kinase type 2 beta

  • Positive control
    • Mouse brain and Saos2 cell lysates and human hepatocarcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71823 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab71823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


  • FunctionTogether with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
    Contains 1 PI3K/PI4K domain.
  • Cellular localizationCytoplasm. Membrane. Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610042N09Rik antibody
    • 4933409G22Rik antibody
    • FLJ11105 antibody
    • P4K2B_HUMAN antibody
    • Phosphatidylinositol 4 kinase type 2 beta antibody
    • Phosphatidylinositol 4 kinase type II beta antibody
    • Phosphatidylinositol 4-kinase type 2-beta antibody
    • Phosphatidylinositol 4-kinase type II-beta antibody
    • PI4K 2 beta antibody
    • PI4K 2b antibody
    • PI4K II beta antibody
    • PI4K IIb antibody
    • Pi4k2b antibody
    • PI4KII-BETA antibody
    • PI4KIIb antibody
    • PIK42B antibody
    • Type II phosphatidylinositol 4 kinase beta isoform antibody
    see all

Anti-PI 4 Kinase type 2 beta antibody - C-terminal images

  • All lanes : Anti-PI 4 Kinase type 2 beta antibody - C-terminal (ab71823) at 1/100 dilution

    Lane 1 : mouse brain lysate
    Lane 2 : Saos2 cell lysate

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • ab71823 at 1/50 dilution, staining PI 4 Kinase type 2 beta in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References for Anti-PI 4 Kinase type 2 beta antibody - C-terminal (ab71823)

ab71823 has not yet been referenced specifically in any publications.

Product Wall

Cross-reactivity with PI4K2A has not been tested but based on sequence homology with ab71823 immunogen (11%) it does not cross-react with PI4K2A.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (multiple myeloma)
Total protein in input 300 µg
Specification multiple myeloma
Immuno-precipitation step Protein A

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Submitted Jan 19 2011