Recombinant
RabMAb

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Overview

  • Product name
    Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]
    See all PI3 Kinase p110 beta primary antibodies
  • Description
    Rabbit monoclonal [EPR5515(2)] to PI3 Kinase p110 beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • MCF-7, Jurkat, K562 and 293T cell lysates; Human brain tissue; 293 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 123 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/75.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

Flow Cyt 1/20.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/150.

For unpurified use at 1/250 - 1/500.

Target

  • Function
    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
  • Tissue specificity
    Expressed ubiquitously.
  • Pathway
    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similarities
    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
    • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
    • DKFZp779K1237 antibody
    • MGC133043 antibody
    • OTTHUMP00000216901 antibody
    • OTTHUMP00000216904 antibody
    • p110 BETA antibody
    • p110Beta antibody
    • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
    • PI3 kinase p110 subunit beta antibody
    • PI3-kinase subunit beta antibody
    • PI3K antibody
    • PI3K beta antibody
    • PI3K-beta antibody
    • PI3Kbeta antibody
    • PI3KCB antibody
    • PIK3C1 antibody
    • Pik3cb antibody
    • PK3CB_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns-3-kinase subunit beta antibody
    • PtdIns-3-kinase subunit p110-beta antibody
    see all

Images

  • Overlay histogram showing SH-SY5Y cells stained with ab131260 (red line) at 1/20 dilution. The cells were fixed with 2% Paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • ab151549 staining PI3 Kinase p110 beta in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/75). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

    Lane 1 : Rat Brain Lysate
    Lane 2 : Mouse Brain Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 123 kDa

  • Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/5000 dilution + K562 Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 123 kDa
    Observed band size: 110 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

    Lane 1 : 293 Cell Lysate
    Lane 2 : Jurket Cell Lysate
    Lane 3 : MCF-7 Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 123 kDa
    Observed band size: 110 kDa (why is the actual band size different from the predicted?)

  • Overlay histogram showing HeLa cells stained with unpurified ab151549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151549, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 123 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/50 dilution.

  • Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.

  • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab151549 showing +ve staining.

References

This product has been referenced in:
  • Jia B  et al. The role of miR-766-5p in cell migration and invasion in colorectal cancer. Exp Ther Med 15:2569-2574 (2018). Read more (PubMed: 29456660) »
  • Zhang Y  et al. Effect of Tetramethylpyrazine on Atherosclerosis and SCAP/SREBP-1c Signaling Pathway in ApoE(-/-) Mice Fed with a High-Fat Diet. Evid Based Complement Alternat Med 2017:3121989 (2017). WB ; Mouse . Read more (PubMed: 28491104) »

See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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