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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab151549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 123 kDa.
For unpurified use at 1/1000 - 1/10000.
For IHC antigen retrieval - See protocols http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.
For unpurified use at 1/50 - 1/100.
For unpurified use at 1/100 - 1/1000.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
For unpurified use at 1/250 - 1/500.
Overlay histogram showing SH-SY5Y cells stained with ab131260 (red line) at 1/20 dilution. The cells were fixed with 2% Paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.
ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.
ab151549 staining PI3 Kinase p110 beta in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/75). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Overlay histogram showing HeLa cells stained with unpurified ab151549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151549, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/50 dilution.
Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.
Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using unpurified ab151549 showing +ve staining.
Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151549 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using unpurified ab151549 showing +ve staining.
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151549 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab151549 showing +ve staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"