Validated using a knockout cell line
Recombinant
RabMAb

Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)

Overview

  • Product name
    Anti-PI3 Kinase p110 beta antibody [Y384]
    See all PI3 Kinase p110 beta primary antibodies
  • Description
    Rabbit monoclonal [Y384] to PI3 Kinase p110 beta
  • Specificity
    ab32569 recognises PI3-Kinases (PI3-Ks).
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A synthetic peptide of human PI3-K p110 beta.

  • Positive control
    • Jurkat cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 123 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function
      Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
    • Tissue specificity
      Expressed ubiquitously.
    • Pathway
      Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • Sequence similarities
      Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
      • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
      • DKFZp779K1237 antibody
      • MGC133043 antibody
      • OTTHUMP00000216901 antibody
      • OTTHUMP00000216904 antibody
      • p110 BETA antibody
      • p110Beta antibody
      • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
      • PI3 kinase p110 subunit beta antibody
      • PI3-kinase subunit beta antibody
      • PI3K antibody
      • PI3K beta antibody
      • PI3K-beta antibody
      • PI3Kbeta antibody
      • PI3KCB antibody
      • PIK3C1 antibody
      • Pik3cb antibody
      • PK3CB_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns-3-kinase subunit beta antibody
      • PtdIns-3-kinase subunit p110-beta antibody
      see all

    Images



    • Predicted band size : 123 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: PI3 Kinase p110 beta knockout HAP1 cell lysate (40 µg)
      Lane 3: Jurkat cell lysate (40 µg)
      Lane 4: K562 cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32569 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. Ab32569 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution + Jurkat cell lysate

      Predicted band size : 123 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Caohuy H  et al. Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of ?F508-cystic fibrosis transmembrane conductance regulator (?F508-CFTR). J Biol Chem 289:35953-68 (2014). Read more (PubMed: 25384981) »
    • Shugg RP  et al. Effects of isoform-selective phosphatidylinositol 3-kinase inhibitors on osteoclasts: actions on cytoskeletal organization, survival, and resorption. J Biol Chem 288:35346-57 (2013). WB ; Mouse . Read more (PubMed: 24133210) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry regarding ab55593 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    I would like to reassure you that our Abpromise ap...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cancer cells)
    Loading amount
    15 µg
    Specification
    cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jun 07 2007

    Thank you for your enquiry. I am sorry but the sequence of the immunogen remains proprietary information. Rabbit monoclonal [Y384] to PI3 Kinase p110 beta (ab32569) has not been tested against the delta form of the protein. However, there appears t...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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