The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
RelevanceTogether with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) PI4K2A contributes to the overall PI4 kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells
Cellular localizationCytoplasm. Membrane. Note: Found in subdomains of the plasma membrane termed non caveolar membrane rafts.