Anti-PI4K2A antibody - N-terminal (ab71824)

Overview

  • Product nameAnti-PI4K2A antibody - N-terminal
    See all PI4K2A primary antibodies
  • Description
    Rabbit polyclonal to PI4K2A - N-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human PI4K2A conjugated to KLH

  • Positive control
    • HL60 cell lysate. Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • RelevanceTogether with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) PI4K2A contributes to the overall PI4 kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells
  • Cellular localizationCytoplasm. Membrane. Note: Found in subdomains of the plasma membrane termed non caveolar membrane rafts.
  • Database links
  • Alternative names
    • Phosphatidylinositol 4 kinase type 2 alpha antibody
    • Phosphatidylinositol 4 kinase type II alpha antibody
    • PI4KII antibody

Anti-PI4K2A antibody - N-terminal images

  • Anti-PI4K2A antibody - N-terminal (ab71824) at 1/100 dilution + HL60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab71824 at 1/50 dilution, staining PI4K2A in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-PI4K2A antibody - N-terminal (ab71824)

ab71824 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 mouse fibroblast cell line)
Loading amount 40 µg
Specification NIH 3T3 mouse fibroblast cell line
Treatment 48 hs-serum deprived
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. GERMAN GIL

Verified customer

Submitted Sep 22 2011

Thank you for your reply and further enquiry. I can confirm that only four nonconsecutive amino acids of the immunogenic sequence for ab71824 PI4K2A antibody are identical to PI4K2B. Although this has not been tested in the laboratory, it is unlike...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (multiple myeloma)
Total protein in input 300 µg
Specification multiple myeloma
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Jan 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"