• Product nameAnti-PIAS1 antibody
    See all PIAS1 primary antibodies
  • Description
    Rabbit polyclonal to PIAS1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the internal sequence of Human PIAS1.

  • Positive control
    • MDA-MB-435 cell extract and Human lung carcinoma tissue.



Our Abpromise guarantee covers the use of ab58403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration.


  • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
  • Tissue specificityExpressed in numerous tissues with highest level in testis.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • DomainThe LXXLL motif is a transcriptional coregulator signature.
    The SP-RING-type domain is required for promoting EKLF sumoylation.
  • Post-translational
    Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
  • Cellular localizationNucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • AR interacting protein antibody
    • DDXBP1 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
    • DEAD/H box binding protein 1 antibody
    • DEAD/H box-binding protein 1 antibody
    • E3 SUMO-protein ligase PIAS1 antibody
    • GBP antibody
    • Gu binding protein antibody
    • Gu-binding protein antibody
    • GU/RH-II antibody
    • Pias1 antibody
    • PIAS1_HUMAN antibody
    • Protein inhibitor of activated STAT protein 1 antibody
    • Protein inhibitor of activated STAT, 1 antibody
    • RNA helicase II binding protein antibody
    • RNA helicase II-binding protein antibody
    • Zinc finger, MIZ-type containing 3 antibody
    • ZMIZ3 antibody
    see all

Anti-PIAS1 antibody images

  • All lanes : Anti-PIAS1 antibody (ab58403) at 1/500 dilution

    Lane 1 : MDA-MB-435 cell extract with no immunizing peptide
    Lane 2 : MDA-MB-435 cell extract with immunizing peptide

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • ab58403, at 1/50 dilution, staining human PIAS1 in lung carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin-embedded tissue.

References for Anti-PIAS1 antibody (ab58403)

This product has been referenced in:
  • Trzcinska-Daneluti AM  et al. High-content functional screen to identify proteins that correct F508del-CFTR function. Mol Cell Proteomics 8:780-90 (2009). WB ; Human . Read more (PubMed: 19088066) »

See 1 Publication for this product

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