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Synthetic peptide derived from within residues 500 - 600 of Human PIAS1.
(Peptide available as ab32218.)
Our Abpromise guarantee covers the use of ab32219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/2000. Detects a band of approximately 81 kDa (predicted molecular weight: 72 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration.|
This image is courtesy of an Abreview submitted by Min young Kim
Image courtesy of Human Protein Atlas
ab32219 staining PIAS1 in human stomach. Paraffin embedded human stomach tissue was incubated with ab32219 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab32219 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
|ab32219 recognizes the full length tagged recombinant protein which has an expected molecular weight of 98 kDa.|