• Product nameAnti-PIAS1 antibody [EPR2580(2)]
    See all PIAS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2580(2)] to PIAS1
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PIAS1 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


    Alternative versions available:

    Anti-PIAS1 antibody (Alexa Fluor® 647) [EPR2580(2)] (ab203328)



Our Abpromise guarantee covers the use of ab109388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
    • Tissue specificityExpressed in numerous tissues with highest level in testis.
    • PathwayProtein modification; protein sumoylation.
    • Sequence similaritiesBelongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • DomainThe LXXLL motif is a transcriptional coregulator signature.
      The SP-RING-type domain is required for promoting EKLF sumoylation.
    • Post-translational
      Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
    • Cellular localizationNucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • AR interacting protein antibody
      • DDXBP1 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
      • DEAD/H box binding protein 1 antibody
      • DEAD/H box-binding protein 1 antibody
      • E3 SUMO-protein ligase PIAS1 antibody
      • GBP antibody
      • Gu binding protein antibody
      • Gu-binding protein antibody
      • GU/RH-II antibody
      • Pias1 antibody
      • PIAS1_HUMAN antibody
      • Protein inhibitor of activated STAT protein 1 antibody
      • Protein inhibitor of activated STAT, 1 antibody
      • RNA helicase II binding protein antibody
      • RNA helicase II-binding protein antibody
      • Zinc finger, MIZ-type containing 3 antibody
      • ZMIZ3 antibody
      see all

    Anti-PIAS1 antibody [EPR2580(2)] images

    • Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
      Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution

      Lane 1 : Recombinant Human PIAS1 protein (ab152888)
      Lane 2 : Recombinant Human PIAS1 protein (ab152888)

      Lysates/proteins at 0.01 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 72 kDa
      Observed band size : 98 kDa (why is the actual band size different from the predicted?)

      Exposure time:

      Lane 1: 10 seconds.

      Lane 2: 1 minute.


      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : 392T cell lysates
      Lane 4 : Daudi cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 72 kDa
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

    This product has been referenced in:
    • Du L  et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun 7:12326 (2016). IHC-P ; Human . Read more (PubMed: 27465491) »
    • Campia I  et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS One 10:e0126159 (2015). WB . Read more (PubMed: 25955018) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification HeLa
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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    Submitted Mar 31 2017