Overview

  • Product nameAnti-PIAS2 antibody
    See all PIAS2 primary antibodies
  • Description
    Goat polyclonal to PIAS2
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    KEAMKVSSQPCTKIE

    , corresponding to internal sequence amino acids 431-445 of Human PIAS2 (NP_004662.2).

  • Positive control
    • Human kidney, prostate and testis tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab115866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
  • Tissue specificityMainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • Developmental stageIsoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.
  • DomainThe LXXLL motif is a transcriptional coregulator signature.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationNucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen receptor interacting protein 3 antibody
    • Androgen receptor-interacting protein 3 antibody
    • ARIP3 antibody
    • DAB2 interacting protein antibody
    • DAB2-interacting protein antibody
    • DIP antibody
    • E3 SUMO protein ligase PIAS2 antibody
    • E3 SUMO-protein ligase PIAS2 antibody
    • MIZ antibody
    • Miz1 antibody
    • Msx interacting zinc finger protein antibody
    • Msx-interacting zinc finger protein antibody
    • PIAS NY protein antibody
    • PIAS-NY protein antibody
    • PIAS2 antibody
    • PIAS2_HUMAN antibody
    • PIASX antibody
    • PIASX-ALPHA antibody
    • PIASX-BETA antibody
    • Protein inhibitor of activated STAT x antibody
    • Protein inhibitor of activated STAT, 2 antibody
    • Protein inhibitor of activated STAT2 antibody
    • SIZ2 antibody
    • Zinc finger, MIZ-type containing 4 antibody
    • ZMIZ4 antibody
    see all

Anti-PIAS2 antibody images

  • ab115866 at 3.75ug/ml staining PIAS2 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115866 at 3.75ug/ml staining PIAS2 in Formalin-fixed, Paraffin-embedded Human prostate tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115866 at 3.75ug/ml staining PIAS2 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-PIAS2 antibody (ab115866)

ab115866 has not yet been referenced specifically in any publications.

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