Recombinant Anti-PIAS2 antibody [EPR2582(2)] (ab126601)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2582(2)] to PIAS2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PIAS2 antibody [EPR2582(2)]
See all PIAS2 primary antibodies -
Description
Rabbit monoclonal [EPR2582(2)] to PIAS2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PIAS2 aa 100-200. The exact sequence is proprietary.
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Positive control
- HepG2 whole cell lysate (ab7900); Human fetal kidney tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2582(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 68 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 68 kDa). |
Target
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Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. -
Tissue specificity
Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger. -
Developmental stage
Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis. -
Domain
The LXXLL motif is a transcriptional coregulator signature. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 9063 Human
- Entrez Gene: 17344 Mouse
- Entrez Gene: 83422 Rat
- Omim: 603567 Human
- SwissProt: O75928 Human
- SwissProt: Q8C5D8 Mouse
- SwissProt: Q6AZ28 Rat
- Unigene: 657844 Human
see all -
Alternative names
- Androgen receptor interacting protein 3 antibody
- Androgen receptor-interacting protein 3 antibody
- ARIP3 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab126601 has been referenced in 2 publications.
- Li C et al. Quantitative SUMO proteomics identifies PIAS1 substrates involved in cell migration and motility. Nat Commun 11:834 (2020). PubMed: 32047143
- Sha Z et al. Inhibiting ubiquitination causes an accumulation of SUMOylated newly synthesized nuclear proteins at PML bodies. J Biol Chem 294:15218-15234 (2019). PubMed: 31285264