Recombinant
RabMAb

Anti-PIAS2 antibody [EPR2582(2)] (ab126601)

Overview

  • Product name
    Anti-PIAS2 antibody [EPR2582(2)]
    See all PIAS2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2582(2)] to PIAS2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PIAS2.

  • Positive control
    • HepG2 cell lysate; Human fetal kidney tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 68 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
    • Tissue specificity
      Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
    • Pathway
      Protein modification; protein sumoylation.
    • Sequence similarities
      Belongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • Developmental stage
      Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.
    • Domain
      The LXXLL motif is a transcriptional coregulator signature.
    • Post-translational
      modifications
      Sumoylated.
    • Cellular localization
      Nucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity).
    • Information by UniProt
    • Database links
    • Alternative names
      • Androgen receptor interacting protein 3 antibody
      • Androgen receptor-interacting protein 3 antibody
      • ARIP3 antibody
      • DAB2 interacting protein antibody
      • DAB2-interacting protein antibody
      • DIP antibody
      • E3 SUMO protein ligase PIAS2 antibody
      • E3 SUMO-protein ligase PIAS2 antibody
      • MIZ antibody
      • Miz1 antibody
      • Msx interacting zinc finger protein antibody
      • Msx-interacting zinc finger protein antibody
      • PIAS NY protein antibody
      • PIAS-NY protein antibody
      • PIAS2 antibody
      • PIAS2_HUMAN antibody
      • PIASX antibody
      • PIASX-ALPHA antibody
      • PIASX-BETA antibody
      • Protein inhibitor of activated STAT x antibody
      • Protein inhibitor of activated STAT, 2 antibody
      • Protein inhibitor of activated STAT2 antibody
      • SIZ2 antibody
      • Zinc finger, MIZ-type containing 4 antibody
      • ZMIZ4 antibody
      see all

    Images

    • All lanes : Anti-PIAS2 antibody [EPR2582(2)] (ab126601) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 68 kDa

    References

    ab126601 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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