Overview

  • Product nameAnti-PIAS3 antibody
    See all PIAS3 primary antibodies
  • Description
    Rabbit polyclonal to PIAS3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Cow, Dog, Pig, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human PIAS3.

  • Positive control
    • This antibody gave a positive signal in both HepG2 and Caco2 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab105178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).

Target

  • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
  • Tissue specificityWidely expressed.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • DomainThe PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
    The LXXLL motif is a transcriptional coregulator signature.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 SUMO protein ligase PIAS 3 antibody
    • E3 SUMO protein ligase PIAS3 antibody
    • E3 SUMO-protein ligase PIAS3 antibody
    • FLJ14651 antibody
    • OTTHUMP00000015586 antibody
    • OTTHUMP00000015587 antibody
    • PIAS 3 antibody
    • Pias3 antibody
    • PIAS3 protein antibody
    • PIAS3_HUMAN antibody
    • Protein inhibitor of activated STAT 3 antibody
    • Protein inhibitor of activated STAT protein 3 antibody
    • Protein inhibitor of activated STAT3 antibody
    • Zinc finger MIZ type containing 5 antibody
    • ZMIZ 5 antibody
    • ZMIZ5 antibody
    see all

Anti-PIAS3 antibody images

  • All lanes : Anti-PIAS3 antibody (ab105178) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-PIAS3 antibody (ab105178)

ab105178 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"