Overview

  • Product nameAnti-PIAS3 antibody
    See all PIAS3 primary antibodies
  • Description
    Rabbit polyclonal to PIAS3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP, corresponding to a sequence within C terminal amino acids 541-590 of Human PIAS3

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab22856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
  • Tissue specificityWidely expressed.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • DomainThe PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
    The LXXLL motif is a transcriptional coregulator signature.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 SUMO protein ligase PIAS 3 antibody
    • E3 SUMO protein ligase PIAS3 antibody
    • E3 SUMO-protein ligase PIAS3 antibody
    • FLJ14651 antibody
    • OTTHUMP00000015586 antibody
    • OTTHUMP00000015587 antibody
    • PIAS 3 antibody
    • Pias3 antibody
    • PIAS3 protein antibody
    • PIAS3_HUMAN antibody
    • Protein inhibitor of activated STAT 3 antibody
    • Protein inhibitor of activated STAT protein 3 antibody
    • Protein inhibitor of activated STAT3 antibody
    • Zinc finger MIZ type containing 5 antibody
    • ZMIZ 5 antibody
    • ZMIZ5 antibody
    see all

Anti-PIAS3 antibody images

  • Anti-PIAS3 antibody (ab22856) at 0.004 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000

    Performed under reducing conditions.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
  • ab22856, at a 1/125-250 dilution, staining human PIAS3 in human liver by Immunohistochemistry, Paraffin embedded tissue. Positive stained hepatocytes indicated by arrows. Magnification 400 x.
  • Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells transfected with either control siRNA or siRNAs targeting PIAS3. Complexes of ErbB4 and PIAS3 were visualized with antibodies against ErbB4 and PIAS3 (ab22856) using in situ PLA. Each red dot represents a single interaction. The nuclei were stained with DAPI (blue).

References for Anti-PIAS3 antibody (ab22856)

This product has been referenced in:
  • Sundvall M  et al. Protein Inhibitor of Activated STAT3 (PIAS3) Protein Promotes SUMOylation and Nuclear Sequestration of the Intracellular Domain of ErbB4 Protein. J Biol Chem 287:23216-26 (2012). ICC/IF . Read more (PubMed: 22584572) »
  • Borghouts C  et al. The intracellular delivery of a recombinant peptide derived from the acidic domain of PIAS3 inhibits STAT3 transactivation and induces tumor cell death. Mol Cancer Res 8:539-53 (2010). WB ; Mouse . Read more (PubMed: 20371673) »

See all 3 Publications for this product

Product Wall

Muchas gracias por tu respuesta.

Si te corre prisa, lo mejor es que pidáis como dices ab22856. Este anticuerpo esta en stock, y en uno o dos días laborables deberíais recibirlo. Os mandaremos un mail automático con...

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Thank you for your enquiry. The immunogen for this antibody is 100% homologous with mouse. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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Thank you for your enquiry and patience. The region of PIAS3 from which the peptide was chosen was: LSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDALGHFFQY (from AA residue 499 to AA residue 553) If you have any additional questions, please contact...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"