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Western blot - PIAS3 antibody (ab22856)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS3 antibody (ab22856)
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Product Name
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PIAS3 antibody
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See all PIAS3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PIAS3
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Immunogen
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Synthetic peptide: HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP, corresponding to a sequence within C terminal amino acids 541-590 of Human PIAS3
BLAST 'HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate.
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Cellular localization
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Mostly nuclear, in nuclear speckles.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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PIAS3 is a member of the protein inhibitor of activated STAT (PIAS) family. It also activates TGF-beta/Smad transcriptional responses. Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PIAS3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PIAS3 antibody images, references, products related to ab22856 and other tools.
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PIAS3 antibody images:
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 Western blot - PIAS3 antibody (ab22856)
PIAS3 antibody (ab22856) at 0.004 µg/ml + HepG2 cell lysate
Secondary
HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
Performed under reducing conditions.
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS3 antibody (ab22856)
ab22856, at a 1/125-250 dilution, staining human PIAS3 in human liver by Immunohistochemistry, Paraffin embedded tissue. Positive stained hepatocytes indicated by arrows. Magnification 400 x.
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References for PIAS3 antibody (ab22856)
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This product has been used in:
Lyst MJ et al. Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. EMBO J 25:5317-28 (2006). WB; Human. PubMed: 17066076
If you publish research using ab22856 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PIAS3
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PIAS3 antibody - more information
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Customer reviews (feedback) regarding PIAS3 antibody |
Customer FAQs regarding PIAS3 antibody |
Protocols for PIAS3 antibody |
Price and availability of products related to PIAS3 antibody |
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PIAS3 antibody - related products:
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PIAS3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PIAS3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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