Overview

  • Product nameAnti-PIAS3 antibody
    See all PIAS3 primary antibodies
  • Description
    Mouse monoclonal to PIAS3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: PPTKKHCSVT SAAIPALPGS KGVLTSGHQP SSVLRSPAMG TLGGDFLSSL PLHEYPPAFP LGADIQGLDL FSFLQTESQH YGPSVITSLD EQDALGHF, corresponding to amino acids 453-551 of Human PIAS3

Properties

Applications

Our Abpromise guarantee covers the use of ab56698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
    • Tissue specificityWidely expressed.
    • PathwayProtein modification; protein sumoylation.
    • Sequence similaritiesBelongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • DomainThe PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
      The LXXLL motif is a transcriptional coregulator signature.
    • Post-translational
      modifications
      Sumoylated.
    • Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 SUMO protein ligase PIAS 3 antibody
      • E3 SUMO protein ligase PIAS3 antibody
      • E3 SUMO-protein ligase PIAS3 antibody
      • FLJ14651 antibody
      • OTTHUMP00000015586 antibody
      • OTTHUMP00000015587 antibody
      • PIAS 3 antibody
      • Pias3 antibody
      • PIAS3 protein antibody
      • PIAS3_HUMAN antibody
      • Protein inhibitor of activated STAT 3 antibody
      • Protein inhibitor of activated STAT protein 3 antibody
      • Protein inhibitor of activated STAT3 antibody
      • Zinc finger MIZ type containing 5 antibody
      • ZMIZ 5 antibody
      • ZMIZ5 antibody
      see all

    Anti-PIAS3 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56698 PIAS3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-PIAS3 antibody (ab56698)

    ab56698 has not yet been referenced specifically in any publications.

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