• Product name
  • Description
    Rabbit polyclonal to PIAS3
  • Specificity
    Detects endogenous levels of total PIAS3 protein
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from Internal of human PIAS3.

  • Positive control
    • Human lung carcinoma tissue 293 cells treated with UV (5 mins)



Our Abpromise guarantee covers the use of ab58406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
IP Use at an assay dependent concentration.


  • Function
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
  • Tissue specificity
    Widely expressed.
  • Pathway
    Protein modification; protein sumoylation.
  • Sequence similarities
    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • Domain
    The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
    The LXXLL motif is a transcriptional coregulator signature.
  • Post-translational
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 SUMO protein ligase PIAS 3 antibody
    • E3 SUMO protein ligase PIAS3 antibody
    • E3 SUMO-protein ligase PIAS3 antibody
    • FLJ14651 antibody
    • OTTHUMP00000015586 antibody
    • OTTHUMP00000015587 antibody
    • PIAS 3 antibody
    • Pias3 antibody
    • PIAS3 protein antibody
    • PIAS3_HUMAN antibody
    • Protein inhibitor of activated STAT 3 antibody
    • Protein inhibitor of activated STAT protein 3 antibody
    • Protein inhibitor of activated STAT3 antibody
    • Zinc finger MIZ type containing 5 antibody
    • ZMIZ 5 antibody
    • ZMIZ5 antibody
    see all

Anti-PIAS3 antibody images

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab58406 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
  • All lanes : Anti-PIAS3 antibody (ab58406) at 1/500 dilution

    Lane 1 : 293 cell extract treated with UV (5 min)
    Lane 2 : 293 cell extract treated with UV (5 min) with peptide

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

References for Anti-PIAS3 antibody (ab58406)

This product has been referenced in:
  • Starsíchová A  et al. TGF-beta1 suppresses IL-6-induced STAT3 activation through regulation of Jak2 expression in prostate epithelial cells. Cell Signal 22:1734-44 (2010). WB ; Human . Read more (PubMed: 20603212) »

See 1 Publication for this product

Product Wall

Te agradezco mucho que nos hayáis enviado el cuestionario relleno, así como las imágenes. Revisando el protocolo todo parece correcto, y me temo que no tengo ninguna sugerencia que hacer para mejorarlo. Es posible que el lote concr...

Read More
Rat Cell lysate - whole cell (Rat Basophilic Leukemia)
Total protein in input
6e+006 cells
Rat Basophilic Leukemia
Immuno-precipitation step
Protein A/G

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Verified customer

Submitted Jun 30 2011


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