Overview

  • Product nameAnti-PIAS3 antibody [PIA3]
    See all PIAS3 primary antibodies
  • Description
    Mouse monoclonal [PIA3] to PIAS3
  • SpecificityThis antibody recognized PIAS3.
  • Tested applicationsSuitable for: ELISA, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    GGALREGHGGPLPS

    , corresponding to amino acids 591-604 of Human PIAS3

  • Positive control
    • HepG2 total cell extract.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC
WB
  • Application notesELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 68 and additional band at 44 kDa may be present (predicted molecular weight: 67 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
    • Tissue specificityWidely expressed.
    • PathwayProtein modification; protein sumoylation.
    • Sequence similaritiesBelongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • DomainThe PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
      The LXXLL motif is a transcriptional coregulator signature.
    • Post-translational
      modifications
      Sumoylated.
    • Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 SUMO protein ligase PIAS 3 antibody
      • E3 SUMO protein ligase PIAS3 antibody
      • E3 SUMO-protein ligase PIAS3 antibody
      • FLJ14651 antibody
      • OTTHUMP00000015586 antibody
      • OTTHUMP00000015587 antibody
      • PIAS 3 antibody
      • Pias3 antibody
      • PIAS3 protein antibody
      • PIAS3_HUMAN antibody
      • Protein inhibitor of activated STAT 3 antibody
      • Protein inhibitor of activated STAT protein 3 antibody
      • Protein inhibitor of activated STAT3 antibody
      • Zinc finger MIZ type containing 5 antibody
      • ZMIZ 5 antibody
      • ZMIZ5 antibody
      see all

    References for Anti-PIAS3 antibody [PIA3] (ab59547)

    ab59547 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"