1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Cellular localizationPIAS3: Nucleus speckle.
PIAS1: Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
PIAS2: Nucleus speckle. Nucleus > PML body. Colocalizes at least partially with promyelocytic leukemia nuclear bodies.
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling PIAS3 + PIAS1 + PIAS2 with purified ab77231 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.