Overview

  • Product name
  • Description
    Rabbit polyclonal to PICALM
  • Specificity
    At least three isoforms of PICALM are known to exist; ab106409 will recognize all three.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).

  • Positive control
    • EL4 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.

Target

  • Function
    Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
  • Tissue specificity
    Expressed in all tissues examined.
  • Involvement in disease
    Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
  • Sequence similarities
    Contains 1 ENTH (epsin N-terminal homology) domain.
  • Cellular localization
    Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALM antibody
    • Clathrin assembly lymphoid myeloid leukemia antibody
    • Clathrin assembly lymphoid myeloid leukemia protein antibody
    • CLTH antibody
    • LAP antibody
    • Phosphatidylinositol-binding clathrin assembly protein antibody
    • PICAL_HUMAN antibody
    • PICALM antibody
    see all

Images

  • Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.

  • ICC/IF image of PICALM staining on blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)

  • All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate with immunizing peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 71 kDa

References

ab106409 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (Blood smear)
Specification
Blood smear
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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