Overview

  • Product name
  • Description
    Rabbit polyclonal to PICALM
  • Specificity
    At least three isoforms of PICALM are known to exist; ab106409 will recognize all three.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).

  • Positive control
    • EL4 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.

Target

  • Function
    Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
  • Tissue specificity
    Expressed in all tissues examined.
  • Involvement in disease
    Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
  • Sequence similarities
    Contains 1 ENTH (epsin N-terminal homology) domain.
  • Cellular localization
    Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALM antibody
    • Clathrin assembly lymphoid myeloid leukemia antibody
    • Clathrin assembly lymphoid myeloid leukemia protein antibody
    • CLTH antibody
    • LAP antibody
    • Phosphatidylinositol-binding clathrin assembly protein antibody
    • PICAL_HUMAN antibody
    • PICALM antibody
    see all

Anti-PICALM antibody images

  • Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.

  • ICC/IF image of PICALM staining on blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)

  • All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate with immunizing peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 71 kDa

References for Anti-PICALM antibody (ab106409)

ab106409 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (Blood smear)
Specification
Blood smear
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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