Anti-Piccolo antibody (ab110427)
Key features and details
- Rabbit polyclonal to Piccolo
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Piccolo antibody
See all Piccolo primary antibodies -
Description
Rabbit polyclonal to Piccolo -
Host species
Rabbit -
Specificity
No cross-reactivity to Bassoon. Multiple isoforms can be detected. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Piccolo.
Database link: Q9Y6V0 -
Positive control
- Mouse and Rat brain lysate; Neuromuscular junction muscle samples from adult mouse.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Constituent: PBS
50% Glycerol
0.09% Sodium Azide -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 567 kDa. For large isoforms reduce dilution.
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IHC-P |
1/400.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 567 kDa. For large isoforms reduce dilution. |
IHC-P
1/400. |
Target
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Function
May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. -
Sequence similarities
Contains 2 C2 domains.
Contains 1 PDZ (DHR) domain. -
Domain
C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change. -
Cellular localization
Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions. - Information by UniProt
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Database links
- Entrez Gene: 26875 Mouse
- Entrez Gene: 56768 Rat
- SwissProt: Q9QYX7 Mouse
- SwissProt: Q9JKS6 Rat
- Unigene: 146275 Mouse
- Unigene: 53925 Rat
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Alternative names
- ACZ antibody
- Aczonin antibody
- Brain-derived HLMN protein antibody
see all
Images
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All lanes : Anti-Piccolo antibody (ab110427) at 1 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 567 kDa -
ab110427 at 1/400 staining Piccolo in neuromuscular junction of whole muscle sample from adult mouse by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Secondary used was Alexa Fluor® 488 goat anti-mouse IgG, IgM (H+L). Red staining was an antibody directed against BTX.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab110427 has been referenced in 1 publication.
- Song SY et al. Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function. Front Neurol 9:465 (2018). PubMed: 30061854