Overview

  • Product name
  • Description
    Rabbit polyclonal to Piccolo
  • Specificity
    No cross-reactivity to Bassoon. Multiple isoforms can be detected.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Human Piccolo protein (BC001304.1).

  • Positive control
    • Mouse and Rat brain lysate; Nuromuscular junction muscle samples from adult mouse.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Constituent: PBS
    Note: 50% Glycerol 0.09% Sodium Azide
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 567 kDa. For large isoforms reduce dilution.
IHC-P 1/400.

Target

  • Function
    May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
  • Sequence similarities
    Contains 2 C2 domains.
    Contains 1 PDZ (DHR) domain.
  • Domain
    C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
  • Cellular localization
    Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACZ antibody
    • Aczonin antibody
    • Brain-derived HLMN protein antibody
    • DKFZp779G1236 antibody
    • KIAA0559 antibody
    • Multidomain presynaptic cytomatrix protein antibody
    • PCLO antibody
    • PCLO_HUMAN antibody
    • Piccolo (presynaptic cytomatrix protein) antibody
    • Piccolo, mouse, homolog of antibody
    • Pico antibody
    • Presynaptic cytomatrix protein antibody
    • Protein piccolo antibody
    see all

Images

  • All lanes : Anti-Piccolo antibody (ab110427) at 1 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 567 kDa
  • ab110427 at 1/400 staining Piccolo in neuromuscular junction of whole muscle sample from adult mouse by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Secondary used was Alexa Fluor® 488 goat anti-mouse IgG, IgM (H+L). Red staining was an antibody directed against BTX.

References

ab110427 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X 100 15 min.
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Username

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Verified customer

Submitted Apr 26 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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