Overview

  • Product nameAnti-PICK1 antibody
    See all PICK1 primary antibodies
  • Description
    Goat polyclonal to PICK1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Horse, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-HYNKLQADPKQ

    , corresponding to internal sequence amino acids 100-110 of Human PICK1 (NP_036539.1; NP_001034672.1; NP_001034673.1)

  • Positive control
    • Mouse and Rat brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionProbable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 AH domain.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications
    Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
  • Cellular localizationCytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ1039K5 antibody
    • MGC15204 antibody
    • OTTHUMP00000028509 antibody
    • PICK 1 antibody
    • Pick1 antibody
    • PICK1_HUMAN antibody
    • PRKCA binding protein antibody
    • PRKCA-binding protein antibody
    • PRKCABP antibody
    • Protein interacting with C kinase 1 antibody
    • Protein interacting with PRKCA antibody
    • Protein interacting with PRKCA 1 antibody
    • Protein kinase C alpha binding protein antibody
    • Protein kinase C-alpha-binding protein antibody
    • Protein that interacts with C kinase 1 antibody
    see all

Anti-PICK1 antibody images

  • Anti-PICK1 antibody (ab77178) at 1 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab77178 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Donkey anti-Goat polyclonal, diluted 1/500.

    See Abreview

References for Anti-PICK1 antibody (ab77178)

ab77178 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cultured Cells (CHO Cells stably transfected with human Pick1)
Specification CHO Cells stably transfected with human Pick1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted May 03 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Stably transfected CHO cells expressing human Pick)
Loading amount 20 µg
Specification Stably transfected CHO cells expressing human Pick
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"