PICK1 overexpression 293T lysate (whole cell) (ab94269)

Overview

  • Product namePICK1 overexpression 293T lysate (whole cell)
  • Description
    PICK1 overexpression lysate
  • General notesab94269 is a 293T cell transfected lysate in which Human PICK1 has been transiently over-expressed using a pCMV-PICK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. PTM: Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. Similarity: Contains 1 AH domain. Contains 1 PDZ (DHR) domain. Tissue specificity: Ubiquitous.

Applications

Our Abpromise guarantee covers the use of ab94269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

PICK1 overexpression 293T lysate (whole cell) images

  • ab94269 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PICK1 antibody (ab56586) at 1/500 dilution

    Lane 1 : PICK1 overexpression 293T lysate (whole cell) (ab94269)
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for PICK1 overexpression 293T lysate (whole cell) (ab94269)

ab94269 has not yet been referenced specifically in any publications.

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