Abcam's β-Hydroxybutyrate Assay Kit (Fluorometric) (ab180876) offers simplicity, enhanced sensitivity, and can be adapted to high-throughput applications. The assay is based on enzymatic oxidation of β-HB that results in generation of fluorescent signal (Ex/Em = 535/587 nm) which is directly proportional to the amount of β-HB. The kit offers an excellent alternative for measurement of β-HB when biological sample quantities are limited or subketogenic levels are suspected. The assay can detect β-Hydroxybutyrate as low as 4 µM in a variety of biological samples.
β-Hydroxybutyrate (β-HB) is the most abundant of the ketone bodies (~78% of total ketone bodies in blood). Ketoacidosis (high concentrations of the ketone bodies) can be caused by a variety of conditions, such as diabetes, alcoholism and severe starvation. Ketoacidosis can be fatal if not treated. Additionally, ketogenic diets can increase the concentration of ketone bodies slightly in healthy individuals.
|β-Hydroxybutyrate Assay Buffer||1 x 25ml|
|β-Hydroxybutyrate Enzyme Mix (Lyophilized)||Green||1 vial|
|β-Hydroxybutyrate Standard (Lyophilized)||Yellow||1 vial|
|β-Hydroxybutyrate Substrate Mix (Lyophilized)||Red||1 vial|
|PicoProbe||Blue||1 x 400µl|
Our Abpromise guarantee covers the use of ab180876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"