Picoprobe β Hydroxybutyrate (beta HB) Assay Kit (Fluorometric) (ab180876)

Overview

  • Product name
    Picoprobe β Hydroxybutyrate (beta HB) Assay Kit (Fluorometric)
    See all Beta Hydroxybutyrate kits
  • Detection method
    Fluorescent
  • Sample type
    Milk, Urine, Serum, Plasma, Other biological fluids
  • Assay type
    Quantitative
  • Sensitivity
    < 4 µM
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's β-Hydroxybutyrate Assay Kit (Fluorometric) (ab180876) offers simplicity, enhanced sensitivity, and can be adapted to high-throughput applications. The assay is based on enzymatic oxidation of β-HB that results in generation of fluorescent signal (Ex/Em = 535/587 nm) which is directly proportional to the amount of β-HB. The kit offers an excellent alternative for measurement of β-HB when biological sample quantities are limited or subketogenic levels are suspected. The assay can detect β-Hydroxybutyrate as low as 4 µM in a variety of biological samples.

  • Notes

    β-Hydroxybutyrate (β-HB) is the most abundant of the ketone bodies (~78% of total ketone bodies in blood). Ketoacidosis (high concentrations of the ketone bodies) can be caused by a variety of conditions, such as diabetes, alcoholism and severe starvation. Ketoacidosis can be fatal if not treated. Additionally, ketogenic diets can increase the concentration of ketone bodies slightly in healthy individuals.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    β-Hydroxybutyrate Assay Buffer 1 x 25ml
    β-Hydroxybutyrate Enzyme Mix (Lyophilized) Green 1 vial
    β-Hydroxybutyrate Standard (Lyophilized) Yellow 1 vial
    β-Hydroxybutyrate Substrate Mix (Lyophilized) Red 1 vial
    PicoProbe Blue 1 x 400µl
  • Research areas
  • Relevance
    Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate, acetone, and primarily beta hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
  • Alternative names
    • 3 hydroxybutyric acid
    • beta HB
    • beta Hydroxybutyric acid

Associated products

Applications

Our Abpromise guarantee covers the use of ab180876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Measurement of β-Hydroxybutyrate concentration in human urine (2.5 µL), serum (2.5 µL) & 2% Milk (5 µL). All samples were deproteinized using 10 kDa Spin Column & spiked with known amount of β-Hydroxybutyrate (400 pmol). Assays were performed following the kit protocol.

Protocols

References

This product has been referenced in:
  • Martínez-García G  et al. An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine. Biosensors (Basel) 7:N/A (2017). Read more (PubMed: 29137135) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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