Picoprobe L-Lactate Assay Kit (ab169557)

Overview

  • Product name
    Picoprobe L-Lactate Assay Kit
    See all L-Lactate kits
  • Sample type
    Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    < 0.2 µM
  • Range
    1000 nmol/well - 250000 nmol/well
  • Species reactivity

    Predicted to work with: a wide range of other species, Mammal
  • Product overview

    Picoprobe L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples. In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate. This simple, rapid and high-throughput suitable assay kit is the most sensitive lactate assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.

  • Notes

    Lactate (CH3CH(OH)COO-) plays an important role in many biological processes. Abnormally high concentrations of lactate have been related to diseases such as diabetes, lactic acidosis, etc. L(+)-Lactate is present in blood and is the major lactate stereoisomer formed in human intermediary metabolism. D-Lactate is also present but only at about 1-5% of L(+)-Lactate concentration. Lactate is a significant energy source for living organisms and can be used to generate cellular ATP.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    L(+)-Lactate Standard (100mM) Yellow 1 x 100µl
    Lactate Assay Buffer WM 1 x 25ml
    Lactate Enzyme Mix (lyophilized) Green 1 vial
    Lactate Substrate Mix Red 1 vial
    PicoProbe Blue 1 x 0.4ml
  • Research areas
  • Relevance
    Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).

Applications

Our Abpromise guarantee covers the use of ab169557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Relative signal (RFU) in filtered mouse heart lysate (3.7 mg protein/mL prior to filtration) in different volumes, comparing L-Lactate signals (black) with background reading (white, no enzyme).

  • L-Lactate measured in unfiltered human plasma (dilution range 1:30-1:90) and mouse serum (dilution range 1:2500-1:7500); duplicates +/- SD. The background control levels (no enzyme) were below level of detection.

  • Measurement of Lactate levels in rat liver (1.2 µg), kidney (0.7 µg), muscle (0.45 µg) and in human serum (0.5 µL from 1:10 diluted serum). Assays were performed following kit protocol.
  • Standard curve with background signal subtracted (duplicates; +/- SD).

Protocols

References

ab169557 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Lactate assay on HK-2 cells

Average Good 4/5 (Ease of Use)
Abreviews
I've used this kit to measure Lactate levels in both cells and supernatant.
Cells:
0.5*10^6 cells were sonicated in assay buffer. 10ul from the lysate were loaded on assay wells. readings were about 10 times higher than the highest value of standard curve. further dilution is needed.
Medium:
10ul were loaded directly to assay well (without de-proteinization).
again, values were much higher than standard curve assay and had to be diluted.

Over all, kit was fairly OK and produced reasonable data.
Username

Shiran Udi

Verified customer

Submitted Apr 06 2016

Please click the link below for detailed protocol

http://docs.abcam.com/pdf/cancer/deproteinization_protocol.pdf

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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