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Picoprobe L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples. In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate. This simple, rapid and high-throughput suitable assay kit is the most sensitive lactate assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.
Lactate (CH3CH(OH)COO-) plays an important role in many biological processes. Abnormally high concentrations of lactate have been related to diseases such as diabetes, lactic acidosis, etc. L(+)-Lactate is present in blood and is the major lactate stereoisomer formed in human intermediary metabolism. D-Lactate is also present but only at about 1-5% of L(+)-Lactate concentration. Lactate is a significant energy source for living organisms and can be used to generate cellular ATP.
|L(+)-Lactate Standard (100mM)||Yellow||1 x 100µl|
|Lactate Assay Buffer||WM||1 x 25ml|
|Lactate Enzyme Mix (lyophilized)||Green||1 vial|
|Lactate Substrate Mix||Red||1 vial|
|PicoProbe||Blue||1 x 0.4ml|
Our Abpromise guarantee covers the use of ab169557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Relative signal (RFU) in filtered mouse heart lysate (3.7 mg protein/mL prior to filtration) in different volumes, comparing L-Lactate signals (black) with background reading (white, no enzyme).
L-Lactate measured in unfiltered human plasma (dilution range 1:30-1:90) and mouse serum (dilution range 1:2500-1:7500); duplicates +/- SD. The background control levels (no enzyme) were below level of detection.
Standard curve with background signal subtracted (duplicates; +/- SD).
ab169557 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"