Our Abpromise guarantee covers the use of ab79960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.|
|RIA||Use a concentration of 5 µg/ml.|
|ELISA||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
ab79960 staining Pig Albumin in pig kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/500) for 1 hour at 22°C. An undiluted Alkaline Phosphatase-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab79960 has not yet been referenced specifically in any publications.