Anti-Pig Albumin antibody (ab79960)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Pig Albumin
  • Tested applications
    Suitable for: WB, RIA, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    Pig Albumin purified from pig plasma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
RIA Use a concentration of 5 µg/ml.
ELISA Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Albumin is a soluble, monomeric protein that makes up about half of the blood serum protein. It is a carrier protein for steroids, fatty acids, and thyroid hormones and also plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • ALB antibody
    • Albumin antibody
    • Serum albumin antibody

Anti-Pig Albumin antibody images

  • ab79960 staining Pig Albumin in pig kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/500) for 1 hour at 22°C. An undiluted Alkaline Phosphatase-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Pig Albumin antibody (ab79960)

ab79960 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated
Sample
Pig Tissue sections (kidney and heart)
Specification
kidney and heart
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Username

Oscar Mccook

Verified customer

Submitted Sep 23 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (porine periodontal ligament cells)
Specification
porine periodontal ligament cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% PBST
Blocking step
1% serum + 0.1% Tween-20 +PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 99.9% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 28 2012

Thank you for contacting Abcam.

I have heard back from the lab and they have told me that this antibody has been tested against Human, Mouse, Rat, Monkey, and Canine plasma. As well as Rabbit and Bovine serum. There was a less than 0.01% cros...

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Thank you for your reply. As I mentioned before normally the discount code has to be issued before purchasing the antibody, in this case I have made an exception. To redeem the discount code below, you must first submit an Abreview on our website (plea...

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Thank you for contacting Abcam. The anti-Pig Albumin antibody has not previously been tested in ICC/IF and so we have no standard protocol for this particular antibody, but I have looked over the protocol you sent and have a few questions/tips that may...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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