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|Sample type||Average %||Range|
|Cell culture supernatant||86.23||77% - 94%|
|Serum||106.7||95% - 114%|
|Plasma||88.19||79% - 97%|
Abcam’s IFN gamma Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Pig IFN gamma in serum, plasma (using EDTA and Citrate as an anticoagulant, heparin is not recommended) and cell culture supernatants.
This assay employs an antibody specific for Pig IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig IFN gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|360X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Pig IFN gamma||2 vials|
|IFN gamma Microplate (12 x 8 wells)||1 unit|
|Recombinant Pig IFN gamma Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113353 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113353 has not yet been referenced specifically in any publications.