Overview

  • Product namePig IL-1 beta ELISA Kit
    See all IL1 beta kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.91 89% - 104%
    Serum 97.32 88% - 105%
    Plasma 87.75 80% - 92%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Pig
  • Product overview

    Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.


     


    This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    For higher sensitivity and reproducibility in only 90 minutes, we recommend Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA® range.


     

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all

Associated products

Applications

Our Abpromise guarantee covers the use of ab100754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Pig IL-1 beta ELISA Kit images

  • Representative Standard Curve using ab100754

Protocols

References for Pig IL-1 beta ELISA Kit (ab100754)

ab100754 has not yet been referenced specifically in any publications.

Product Wall

Application ELISA
Sample Pig Serum (cell extracts)
Type of Kit Used ELISA
Specification cell extracts
Did you use the Abcam Kit protocol? Yes
Amount of Sample 100 µg
Positive control standard provided by kit
Username

Dr. Carolina Velez

Verified customer

Submitted Feb 22 2016

Abreview for IL1 beta Pig ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Pig Serum (Blood)
Specification: Blood
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Standards IL1 Beta
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Serum samples were analyzed undiluted, all samples were within the detection range of the kit, and the calibration curve was well adjusted (R2: 0.9992).
Username

Abcam user community

Verified customer

Submitted Mar 26 2013

Thank you for contacting us.

I have received following reply from my colleague in laboratory;

I’m not exactly sure what the customer means by “one recovery at a high concentration” as the Spiking and Recovery QC data...

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Thank you for contacting us.

This kit is developed by one of our collaborator. Our collaborator product as this one has passed strict quality checks we have at Abcam which is why we are selling this kit. The product has been fully tested with ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"