|Sample type||Average %||Range|
|Cell culture supernatant||132.1||123% - 142%|
|Serum||111.7||102% - 121%|
|Plasma||97.02||70% - 126%|
Abcam’s TNF alpha Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Pig TNF-alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Pig TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|600X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Pig TNF alpha||2 vials|
|Recombinant Pig TNF alpha Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
|TNF alpha Microplate (12 x 8 wells)||1 unit|
Our Abpromise guarantee covers the use of ab100756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100756 has not yet been referenced specifically in any publications.