The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
FunctionMay be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).
Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
All lanes : Anti-PIG3 antibody (ab64798) at 1 µg/ml
Lane 1 : Caco 2 whole cell lysate (ab3950) Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 5 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate Lane 6 : Lung (Human) Tissue Lysate Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 8 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 35 kDa Observed band size : 35 kDa
Exposure time : 2 minutes
References for Anti-PIG3 antibody (ab64798)
This product has been referenced in:
Mungamuri SK et al. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene0:N/A (2014).
Read more (PubMed: 25023704) »
Kracikova M et al. A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ20:576-88 (2013).
Read more (PubMed: 23306555) »