Overview

  • Product name
    Anti-PIGA antibody - C-terminal
    See all PIGA primary antibodies
  • Description
    Rabbit polyclonal to PIGA - C-terminal
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PIGA aa 455-484 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P37287

  • Positive control
    • MDA-MB435 cell line lysate; Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170598 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 54 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
  • Pathway
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Involvement in disease
    Paroxysmal nocturnal hemoglobinuria (PNH) [MIM:300818]: A disorder characterized by hemolytic anemia with hemoglobinuria, thromboses in large vessels, and a deficiency in hematopoiesis. Clinical manifestation of red blood cell breakdown with release of hemoglobin into the urine is manifested most prominently by dark-colored urine in the morning. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Multiple congenital anomalies-hypotonia-seizures syndrome 2 (MCAHS2) [MIM:300868]: An X-linked recessive developmental disorder characterized by dysmorphic features, neonatal hypotonia, myoclonic seizures, and variable congenital anomalies involving the central nervous, cardiac, and urinary systems. Most affected individuals die in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Class A GlcNAc inositol phospholipid assembly protein antibody
    • GlcNAc PI synthesis protein antibody
    • GlcNAc-PI synthesis protein antibody
    • GPI3 antibody
    • MCAHS2 antibody
    • Phosphatidylinositol glycan anchor biosynthesis class A antibody
    • Phosphatidylinositol glycan biosynthesis class A protein antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit A antibody
    • Phosphatidylinositol-glycan biosynthesis class A protein antibody
    • PIG A antibody
    • PIG-A antibody
    • Piga antibody
    • PIGA_HUMAN antibody
    see all

Images

  • Anti-PIGA antibody - C-terminal (ab170598) at 1/100 dilution + MDA-MB435 cell line lysate at 35 µg

    Predicted band size : 54 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling PIGA with ab170598 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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