Overview

  • Product name
    Anti-PIGB antibody
  • Description
    Rabbit polyclonal to PIGB
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: QRGTLDVMSH IQKVCYNNPN KSSASIFIMM PCHSTPYYSH VHCPLPMRFL QCPPDLTGKS HYLDEAD, corresponding to amino acids of Human PIGB.

  • Positive control
    • Human rectum tissue; RT 4 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500.

Target

  • Function
    Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.
  • Pathway
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Sequence similarities
    Belongs to the glycosyltransferase 22 family. PIGB subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GPI mannosyltransferase 3 antibody
    • GPI mannosyltransferase III antibody
    • GPI-MT-III antibody
    • Phosphatidylinositol-glycan biosynthesis class B protein antibody
    • PIG-B antibody
    • pigb antibody
    • PIGB_HUMAN antibody
    see all

Images

  • All lanes : Anti-PIGB antibody (ab122603) at 1/100 dilution

    Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
    Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab122603, at 1/20 dilution, staining PIGB in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • All lanes : Anti-PIGB antibody (ab122603) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab122603 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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