Overview

  • Product name
    Anti-PIGF antibody (Biotin)
    See all PiGF primary antibodies
  • Description
    Rabbit polyclonal to PIGF (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab84226 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human PlGF in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions.
  • Tested applications
    Suitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human PlGF

  • Positive control
    • Recombinant human PlGF.

Properties

  • Form
    Lyophilised:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Sandwich ELISA
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well reaction).
    sELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well reaction).
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
    • Pathway
      Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
    • Sequence similarities
      Belongs to the PIGF family.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GPI11 homolog antibody
      • MGC32646 antibody
      • MGC33136 antibody
      • PHOSPHATIDYLINOSITOL GLYCAN antibody
      • Phosphatidylinositol glycan biosynthesis class F protein antibody
      • Phosphatidylinositol-glycan biosynthesis class F protein antibody
      • PIG F antibody
      • PIG-F antibody
      • PIGF antibody
      • PIGF_HUMAN antibody
      • PIGF1 antibody
      see all

    References

    ab84226 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab84226.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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