Overview

  • Product name
  • Description
    Rabbit polyclonal to PIGK
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 amino acids in length) conjugated to KLH, from the N terminal of the first 100 amino acids of Human PIGK.

  • Positive control
    • 293 cell lysate, Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50 - 1/100.

Target

  • Relevance
    PIGK is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
  • Cellular localization
    Endoplasmic reticulum, Endoplasmic reticulum membrane; type I single pass membrane protein
  • Database links
  • Alternative names
    • GPI anchor transamidase antibody
    • GPI transamidase antibody
    • GPI8 antibody
    • Phosphatidylinositol glycan biosynthesis class K protein antibody
    • Phosphatidylinositol glycan class K antibody
    • PIG K antibody
    see all

Images

  • Anti-PIGK antibody (ab38445) at 1/100 dilution + 293 cell lysate

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab38445, at 1/50 dilution, staining PIGK in Human breast carcinoma by Immunohistochemistry, Formalin fixed and Paraffin embedded tissue. ab38445 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

ab38445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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