Purification notesThis antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
1/50 - 1/100.
RelevancePIGK is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
Cellular localizationEndoplasmic reticulum, Endoplasmic reticulum membrane; type I single pass membrane protein
ab38445, at 1/50 dilution, staining PIGK in Human breast carcinoma by Immunohistochemistry, Formalin fixed and Paraffin embedded tissue. ab38445 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
References for Anti-PIGK antibody (ab38445)
has not yet been referenced specifically in any publications.
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