Overview

  • Product name
  • Description
    Rabbit polyclonal to PIGO
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region of Human PIGO

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 74 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • Function
    Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI third mannose which links the GPI-anchor to the C-terminus of the proteins by an amide bond.
  • Pathway
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Sequence similarities
    Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434M222 antibody
    • FLJ00135 antibody
    • FLJ00350 antibody
    • GPI ethanolamine phosphate transferase 3 antibody
    • mFLJ00350 antibody
    • MGC20536 antibody
    • MGC3079 antibody
    • OTTHUMP00000021324 antibody
    • OTTHUMP00000021325 antibody
    • OTTMUSP00000007085 antibody
    • Phosphatidylinositol glycan anchor biosynthesis class O antibody
    • Phosphatidylinositol glycan biosynthesis class O protein antibody
    • Phosphatidylinositol glycan class O antibody
    • Phosphatidylinositol-glycan biosynthesis class O protein antibody
    • PIG O antibody
    • PIG-O antibody
    • PIGO antibody
    • PIGO_HUMAN antibody
    • RP11-182N22.4 antibody
    • RP23-124L1.7 antibody
    see all

Images

  • Anti-PIGO antibody (ab75502) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 74 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

References

ab75502 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75502.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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