Overview

  • Product name
  • Description
    Rabbit polyclonal to PIGQ
  • Specificity
    ab84624 detects isoform 1, 2, and 3.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (PTVLPDRQAG ATTASTGGLA AVFDTVARSE VLFRSDRFDE GPVRLSHWQS) of human PIGQ (NP_004195).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.
  • Pathway
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Sequence similarities
    Belongs to the PIGQ family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Phosphatidylinositol glycan, class Q antibody
    • c407A10.1 antibody
    • GPI1 antibody
    • hGPI1 antibody
    • MGC12693 antibody
    • N-acetylglucosamyl transferase component GPI1 antibody
    • Phosphatidylinositol glycan anchor biosynthesis, class Q antibody
    • Phosphatidylinositol N acetylglucosaminyltransferase subunit Q antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit Q antibody
    • Phosphatidylinositol-glycan biosynthesis class Q protein antibody
    • PIG Q antibody
    • PIG-Q antibody
    • PIGQ antibody
    • PIGQ_HUMAN antibody
    see all

Images

  • Anti-PIGQ antibody (ab84624) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References

ab84624 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84624.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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