Overview

  • Product name
  • Description
    Rabbit polyclonal to PIGS
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 468-517 (AVAAVQKAAK ALALGHLSSA FAASQEAVTS SERAFFDPSL LHLLYFPDDQ) of Mouse PIGS (NP_958808).

  • Positive control
    • Mouse small intestine lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab113817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.
  • Pathway
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Sequence similarities
    Belongs to the PIGS family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686K20216 antibody
    • FLJ45226 antibody
    • GPI transamidase component PIG-S antibody
    • GPI transamidase subunit antibody
    • Phosphatidylinositol glycan anchor biosynthesis class S antibody
    • Phosphatidylinositol glycan class S antibody
    • Phosphatidylinositol-glycan biosynthesis class S protein antibody
    • PIGS antibody
    • PIGS_HUMAN antibody
    see all

Anti-PIGS antibody images

  • Anti-PIGS antibody (ab113817) at 1 µg/ml + Mouse small intestine lysate at 10 µg

References for Anti-PIGS antibody (ab113817)

ab113817 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up