Recombinant
RabMAb

Anti-PIGS antibody [EPR11275(B)] (ab157211)

Overview

  • Product name
    Anti-PIGS antibody [EPR11275(B)]
  • Description
    Rabbit monoclonal [EPR11275(B)] to PIGS
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human PIGS was used as an immunogen (UniProt ID Q96S52).

  • Positive control
    • PC-3, fetal liver, HeLa and A431 lysates; Immunoprecipitation pellet from HeLa cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11275(B)
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab157211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    Images

    • All lanes : Anti-PIGS antibody [EPR11275(B)] (ab157211) at 1/1000 dilution

      Lane 1 : PC-3 cell lysate
      Lane 2 : Fetal liver cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 62 kDa
    • Immunoprecipitation analysis of pellet from HeLa cell lysate labeling PIGS using ab157211 at 1/10 dilution.

    References

    ab157211 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Apr 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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